This is the first study on the inheritance and genetic mapping of resistance to the barley grass stripe rust pathogen (Puccinia striiformis f. sp. pseudohordei – Psph) in bread wheat. Psph, commonly infects barley grass (Hordeum leporinum, H. murinum), but about 10% of commercial barley varieties are also susceptible. We tested over 500 diverse wheat accessions and determined that less than 20% were susceptible at the seedling stage suggesting wheat is an ‘intermediate’ host to Psph. The Australian variety Teal is highly susceptible to Psph at the seedling stage, whereas selections Avocet S and Avocet R are highly resistant and resistant, respectively. We used the Teal/AvocetR doubled haploid (DH) population to characterize the resistance of Avocet R to Psph and determine whether the complementary genes Yr73 and Yr74 (YrA resistance) in Avocet R conferred resistance to Psph. Phenotypic comparison of the Teal/AvocetR DH lines in response to both Psph and Pst showed that all DH lines carrying YrA were also resistant to Psph; however, fewer DH lines were susceptible to Psph suggesting additional resistance genes. Marker-trait association analysis detected three DArT-Seq markers significantly associated with resistance to Psph, two mapping to chromosomes 3DL and 5BL in the same regions as Yr73 and Yr74 and the third mapping to chromosome 4A. Single gene stocks with the 4A gene and combinations of the 5BL and 3DL genes will be used for monitoring avirulence/virulence within Australian Psph population. Genetic analysis of seedling-susceptible T/AvR DH lines as adult plants in the greenhouse determined that Teal and Avocet R each carried at least one APR gene effective against Psph.
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This study reports the inheritance and genetic mapping of YrA seedling resistance to stripe rust in a resistant selection of the Australian spring wheat variety Avocet (AUS20601). Genetic analysis was performed on F2 and F3 generation families derived from crosses between wheat genotypes previously reported to carry the YrA resistance and lines that lack the YrA resistance phenotype. Seedling tests with two Pst pathotypes (104 E137 A- and 108 E141 A-) avirulent with respect to YrA confirmed that the resistance was inherited as two complementary dominant genes. Ninety-two doubled haploid (DH) lines derived from a cross between the Australian cv. Teal (seedling-susceptible) and Avocet R were used to confirm the mode of inheritance of YrA and to develop a DArT-Seq genetic map to locate the components of the YrA resistance. Marker-trait association analysis based on 9,035 DArT-Seq loci mapped the two genes to chromosomes 3DL and 5BL. F2 populations derived from intercrosses of seedling susceptible DH lines that carried each gene (based on marker genotype) reproduced the YrA phenotype and specificity, confirming the complementary resistance gene model. The YrA resistance component loci were designated Yr73 (3DL) and Yr74 (5BL). Candidate single gene reference stocks will be permanently accessioned following cytological analysis to avoid a T5B-7B translocation in Teal relative to Avocet and Chinese Spring.