Displaying 11 - 12 of 12

Characterization of seedling and adult plant resistance to leaf rust in African wheat germplasm

Many of the catalogued leaf rust resistance genes in wheat deployed in agriculture have been overcome by variants of Puccinia triticina (Pt), the causal pathogen of leaf rust. Discovery and characterization of new sources of resistance in various germplasms using multipathotype tests and molecular markers could permit future diversification of the genetic base of leaf rust resistance in wheat. In searching for new sources of leaf rust resistance, 140 wheat lines from 14 African countries were tested with 8 Australian Pt pathotypes. Seedling tests revealed that 41% of the lines were susceptible to all pathotypes, 31% were postulated to carry either one of 10 resistance genes (Lr1, Lr2a, Lr3a, Lr13, Lr18, Lr23, Lr24, Lr26, Lr37 or Lr73) or one of five gene combinations (Lr2a+Lr3a, Lr1+Lr13, Lr1+Lr23, Lr1+Lr13+Lr73 and Lr23+Lr73). Twenty-eight percent of the lines were postulated to carry uncharacterized seedling resistance genes. Based on average coefficients of infection (ACI), 101, 25 and 11 lines showed high (ACI 0-19), moderate (ACI 21-38) and low (ACI 41-56) levels adult plant resistance, respectively, whereas three lines were moderately susceptible to susceptible (ACI 63-76). Genotyping of 74-78 lines that were anticipated to carry APR genes, using the molecular markers: csLV34 (linked to Lr34) and KASP SNP markers SNP1G22 and SNPT10 (linked to Lr46 and Lr67), respectively, revealed the presence of Lr34, Lr46 and Lr67 in 11, 22 and 14 wheat lines, respectively. The identities of the APR in the remaining 22 lines are unknown, and potentially represent new resistance sources. Genetic analyses of these uncharacterized APR sources are underway to select single gene lines and allow fine mapping.

The University of Sydney, Plant Breeding Institute, Australia
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Identifying quantitative trait loci for high-temperature adult-plant resistance to stripe rust in six mapping populations of wheat

High-temperature adult-plant (HTAP) resistance to stripe rust in wheat has proven to be durable. Molecular markers tightly linked to HTAP resistance offer an alternative to phenotypic selection and are useful for pyramiding HTAP resistance genes with other types of resistance. This study assessed HTAP resistance in six diverse mapping populations derived from four types of crosses: 1. Resistant × Susceptible, IDO444 × Rio Blanco (winter wheat RILs) and UI Silver × Shaan 89150 (winter wheat DH lines); 2. Moderately Resistant × Resistant, UI Platinum × SY Capstone (spring wheat DH lines) and UI Stone × IDO686 (spring wheat RILs); 3. Moderately Resistant × Moderately Resistant, UI Stone × Alturas (spring wheat RILs); and 4. Moderately Susceptible × Moderately Susceptible, IDO835 × Moreland (winter wheat, DHLs). Eight QTL significantly associated with HTAP resistance were earlier identified in the IDO444 x Rio Blanco RIL population, including three major QTL (QYrid.ui-2B.1, QYrid.ui-2B.2, and QYrid.ui-4A) and five minor QTL (QYrid.ui-1A, QYrid.ui-3B.1, QYrid.ui-3B.2, QYrid.ui-4B, and QYrid.ui-5B) (Chen et al. 2012, Mol Breeding 29:791–800). These QTL are being validated and novel QTL are being identified in the other five populations. The current study used elite × elite crosses; therefore, the identified QTL may have application in selecting lines with combinations of stripe rust resistance and other superior agronomic traits and perhaps for release as new cultivars.

University of Idaho, USA
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