All BGRI Abstracts

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Breeding Studies of Resistant Varieties to Stripe and Stem Rust Diseases in West Transitional Zone of Turkey

Belen Transitional Zone Agricultural Research Institute, Turkey

The most serious wheat diseases affecting yield and quality in West Transitonal Zone of Turkey is rust diseases. Breeding resistant varieties is the most economic and confident way to struggle with these diseases. In this study, it was aimed to determine the genotypes which are resistant to stripe and stem rust diseases existing in our region and use these genotypes as material in breeding program. The study was carried out in 2014 at natural and artificial epidemic conditions in the experimental field of  Transitional Zone Agricultural Research Institute. The reactions of some wheat genotypes to stripe and stem rust diseases caused by the fungal agents P.striformis ve P.graminis were detected. For this purpose,  310 lines and  18 variety of  bread wheat belonging to Transitional Zone Agricultural Researh Institute were sown in a way 1 m x 1 row. The disease assessments were conducted in May-June 2014 according to the Modified Coob Scale. As a result of infection coefficient observations, it was determined that 49% of the material are resistant to stripe rust and 60% of the material are resistant to stem rust.


Identification of slow stripe rusting wheat genotypes

Saharan Indian Institute of Wheat and Barley Research (IIWBR), India

Stripe or yellow rust is a constraint to wheat production on about 12.8 m ha in the Northern Hills and North Western regions of India. Varieties resistant at the time of release become susceptible usually within a few years due to new pathogen races. The present study conducted in 2013-14 was undertaken to identify slow stripe rusting genotypes among a panel of 192 advanced breeding lines and popular cultivars. All genotypes were planted in two replications and a susceptible control was planted after every 20 plots. The nursery, grown at Karnal, was inoculated with a mixture of prevalent Pst races 78S84 and 46S119. Genotypes were categorized into distinct groups based on area under disease progress curve (AUDPC) values, viz. 22 lines with AUDPC values 1-100, 18 lines with values 101-200, 43 lines with values 201-500, and remaining lines with higher values. Apart from rust-free lines assumed to carry all-stage resistance genes, lines with AUDPC values of less than 500 and having AUDPC values <20% of those of the susceptible check (maximum AUDPC value, 2500) were considered to be slow rusting. Some of the popular cultivars (HS 507, HS 542, WH 1105, HD 3086, DPW 621-50, HD 3059) currently grown in northern India showed slow rusting. The information generated can be utilized in improving the levels of stripe rust resistance in current cultivars.


Gene-assisted selection using a pedigree dissection approach to identify Yr genes in wheat breeding materials

Ghaffary Safiabad Agricultural Research Center, Iran

Yellow rust is the most dangerous of the wheat rusts worldwide. Disease management involves breeding and fungicide application, with the former being more cost effective and environmentally acceptable. Despite the release of numerous yellow rust resistant cultivars in many countries, new aggressive strains inevitably overcome the resistances in a zigzag or ‘boom and bust’manner. For example, Chamran (Attila-50-Y), released in 1997 in Iran, immediately became the most popular cultivar nationally. In 2012-2013, a new aggressive Pst strain overcame the resistance in Chamran as well as Vee/Nac (an early maturity line suitable for the wheat-maize cropping system) in Khuzestan, a major wheat-producing region in the southwest of the country. Evaluations of wheat germplasm at the Safiabad Agricultural Research Center (North Khuzestan) identified 17 completely or partially resistant lines. Pedigree analyses of resistant lines identified Batavia, Genaro 81, Opata, Pastor, Trap and Yaco as possible sources of resistance. Genotype information of these cultivars obtained from the database indicated the presence of Yr33, Yr30+Yr18 and Yr31 in Batavia, Opata and Pastor, respectively. Genaro 81, Trap and Yaco carry Yr18. Currently, F2 populations of 34 crosses of 17 resistant lines to locally adapted cultivars Chamran and Vee/Nac are undergoing field selection in a nursery inoculated with the 2012-2013 aggressive race. The progenies of selected plants will undergo further testing and selected homozygous F3 lines will be genotyped for markers associated with Yr18 (Xgwm295-7D), Yr30 (flanking markers Xgwm533.1 - Xgwm493-3B), Yr31 (Xgwm630/Xgwm374-2B (Lr13/Lr23)) and Yr33 (flanking markers Xgwm111 - Xgwm437-7D).


Genetic relationship between wheat stem rust resistance genes Sr36 and Sr39

Chemayek The University of Sydney, Plant Breeding Institute, Australia

Stem rust resistance genes Sr39 (RL6082) and Sr36 (Cook) were transferred from Aegilops speltoides and Triticum timopheevi to chromosome 2B of wheat. Both genes are located on large translocated segments. Genotypes carrying Sr36 and Sr39 produce infection types (ITs) 0; and 2, respectively, against avirulent pathotypes. This investigation was planned to study the genetic relationship between these genes with the aim of combining them in a single genotype. Seedling tests on RL6082/Cook F3 lines showed complete repulsion linkage [25 Sr39Sr39sr36sr36 (IT2-) : 53 Sr39sr39Sr36sr36 (IT2-, IT0;) : 13 sr39sr39Sr36Sr36 (IT 0;)], and preferential transmission of the Ae. speltoides segment over the T. timopheevi segment was evident from the segregation ratio. The Sr39-carrying translocation was shortened by Niu et al. (2011; Genetics 187: 1011-1021) and the genetic stock carrying the shortest segment was named RWG1. Based on the reported location of Sr39 in the smaller alien segment in RWG1, we predicted that it should recombine with Sr36. F3 lines derived RWG1/Cook were phenotyped for stem rust response at the two-leaf stage and again complete repulsion linkage between Sr39 and Sr36 was observed [23 Sr39Sr39sr36sr36 (IT2-) : 78 Sr39sr39Sr36sr36 (IT0;, IT2-) : 68 sr39sr39Sr36Sr36 (IT 0;)]. In contrast to the cross involving the large Sr39 translocation, preferential transmission of the T. timopheevi segment was observed. These results indicated that a genetic determinant of meiotic drive had been deleted in the shortened Ae. speltoides segment. Genotyping with the co-dominant STS marker rwgs28 matched the phenotypic classification of F3 families. Marker rwgs28 was diagnostic for the Ae. speltoides segment, but the rwgs28 allele amplified in Cook was not T. timopheevi-specific.


The features of wheat rust development and the resistance of local cultivars in Kazakhstan

Koyshibayev Kazakh Research Institute of Plant Protection and Quarantine, Kazakhstan

The climatic conditions of Kazakhstan are suitable to grow the high-quality grain of spring wheat on an area of 12-14 mil.ha. The country’s sharply continental climate limits the wheat yield as well as biotic stresses. Among latter factors, diseases significantly reduce yield up to 25% and more during epyphytoties. In the Northern Kazakhstan the considerable threat for common wheat comes from leaf rust (Puccinia  triticina), stem rust (Puccinia graminis), septoria (Stagonospora nodorum, Septoria tritici), and tan spot (Pyrenophora tritici-repentis); yellow rust (Puccinia striiformis) infects wheat plants in the South and South-east regions, where the winter wheat is more common. Epiphytoties of leaf rust were observed in 2000, 2002, 2005, and 2007. Many years research has led to conclusion that local wheat varieties do not possess resistance to mentioned diseases. Last year screening of 46 cultivars at Kostanay province designated virulence to local pathotypes, except of couple of them (Kazakhstanaskaya 19 and Karabalykskaya 20). Russian varieties (Omskaya 37, Omskaya 39, Omskaya 41, Uralo-sibirskaya, Pamyati Mayestrenka, Lyubava, and Altayskaya zhnitza) demonstrated “slow rusting”. In the period of 2001-2014 the effectiveness to leaf rust was identified using Thatcher isogenic lines under northern Kazakhstan conditions and showed avirulence to local Pt pathotypes in lines carrying Lr9, Lr24, Lr29, Lr35, and Lr37 as well as the pyramid of Lr genes and/or “slow rusting” genes. The essential spread of stem rust was recorded in 2007, 2008, 2013, and 2014. Taking into account the absence of local sources of infection.  In addition, the monitoring of pathogen with use of a set of lines with Sr genes detected the absence of aggressive race within north of Kazakhstan. In order to create resistant cultivars the sources of resistance are recommended to apply from current study.