Seed & Plant Improvement Institute (SPII), AREEO, Karaj, Iran
Ramin Roohparvar, Safarali Safavi, Gholamhossein Ahmadi
In recent years, wheat stem rust, caused by Puccinia graminis f.sp. tritici, has been reconsidered in Iran due to its prevalence and the emergence of the dangerous Ug99 race. This study was conducted to understand pathogenic variation in the population of P. graminis f.sp. tritici, detection of effective genes, and identification of resistance in Iranian commercial wheat cultivars or advanced lines, by planting stem rust trap nurseries under natural disease infection in several regions of Iran during the 2016-2017 cropping season. The trap nursery in each location included 48 wheat lines each carrying a single gene of stem rust (Sr) resistance, seven lines each carrying Sr multigenes, eight additional lines to confirm four Sr genes, 149 commercial wheat cultivars or advanced lines from Iran, plus several susceptible checks. The percentage leaf area affected (disease severity) and infection type were recorded at adult plant stage when disease was well developed on flag leaves of susceptible checks. Results showed presence of virulence for several Sr genes in one or more locations. However, the single genes of Sr13, Sr23, Sr24, and two complex genes of Sr7a+Sr6+Sr12 and Sr6+Sr24+Sr36+Sr1RS-Am were still effective against stem rust in all locations. The results of evaluations of commercial wheat cultivars or advanced lines showed that approximately 16% the genotypes tested including wheat cultivars Gonbad, Shiroudi, Chamran-2, Baharan, Dena, Karkheh, and Arya were resistant in all locations.
Jianping Zhang, Peng Zhang, Robert Park, Narayana Upadhyaya, Robert McIntosh, Sambasivam Periyannan, Brande Wulff, Burkhard Steuernagel, Evans Lagudah
Evolution of rust pathogens continues to pose challenges to global wheat production. Major resistance (R) genes, which encode proteins of the NBS-LRR (Nucleotide-binding site, leucine-rich repeat) family, have been a valuable resource for breeders to minimise yield losses from infection. Many wheat varieties harbor numerous R genes that could be identified and cloned in order to engineer more sustainable disease control. The advent of targeted gene enrichment and next-generation sequencing (NGS) has allowed rapid cloning of specific R genes, thus enhancing efforts to pyramid these genes and investigate their underlying resistance mechanisms. Several R genes present different phenotypes in certain genetic backgrounds, and cloning them would be an important step towards uncovering their interactions. Hybrid necrosis is one such phenotype observed in crosses of wheat genotypes involving the R gene Lr13 and complementary genes, Ne1 and Ne2, occurring in different allelic forms. It was recently concluded that Lr13 and an allele of Ne2 are actually the same gene based on genetic and mutational studies. The capability of Lr13 to confer both leaf rust resistance and hybrid necrosis cannot be answered without first cloning it. The lack of tightly linked markers coupled with the proximal 2BS chromosomal location of Lr13 does not make it easily amenable to map-based cloning. The NGS-based pipeline MutRenSeq (mutagenesis and R-gene enrichment sequencing) was used on EMS (Ethyl methanesulfonate) induced, susceptible Lr13 mutants along with support from comparative genomics to ascertain candidate gene sequences for Lr13, which are at advanced stages of screening and confirmation. Definite proof that a single gene is involved will only come with transformation studies when the cloned Lr13 candidate transformed into a susceptible line confers both a resistance phenotype in the transgenic line and a necrotic phenotype in the offspring of crosses between the transgenic line and a line possessing Ne1.
Sathguru Management Consultants
Kanan,Vijayaraghavan, Vijay, Paranjape, Richa, Kapur, Vignesh, Vilayanur Jayaraman, , , , , , , , , , , , , , , , , , , , , ,
Wheat is one of the most important food crops of the world. India is the second largest producer of wheat, currently producing 95 million tons from about 30 million hectares. Looking ahead to 2050, India needs to constantly increase production to about 150 million tons, to meet the rising population and demand. With area under cultivation having no room for growth, productivity will be the main pillar for growing production. Currently India?s yield of 3.1 t/ha has plenty room for growth as compared to the world leaders such as France (7.5 t/ha), Germany (7.3 t/ha) and UK (6.6 t/ha). Wheat productivity depends on multiple factors, seed being one of the most important.
The current operating environment is characterized by wheat R&D in the country conducted by public institutes, but there are clear signs of an emerging private sector involvement. The government promoting Inter-institutional linkages by way of associating private players in research and seed production.
This study evaluates and reflects on the current situation of the wheat seed sector in India - from research, variety/hybrid development, seed production, indent to distribution, the players involved, the challenges therein, upcoming technologies and the way forward.
National Agronomic Institute of Tunisia
Sana Kamel, Elhem, Elfahem, Wissal Feriani, Hanen Sbei
In order to identify sources of resistance to tan spot caused by Pyrenophora tritici-repentis, 359 local wheat accessions were evaluated for reaction to the Oued-Mliz isolate in controlled conditions and in the field. Two and three assessments were carried out at the seedling and adult stages, respectively. There was a highly significant accession effect and 4.2% of accessions were highly resistant in both controlled conditions and the field. Assessments at the seedling stage were positively correlated with each other, and assessments in the adult stage were also positively correlated. However, assessments at the seedling stage were negatively correlated with those at the adult stage. One hundred and fifty five accessions with known origins (from 15 localities belonging to four districts) were projected on a graph defined by the two axes: reactions at the seedling stage and reactions at the adult stage. After placing the average reactions at the seedling and adult stages on the graph, four groups of accessions were obtained: accessions that were resistant to both stages, accessions that were resistant at the adult stage only, accessions that were resistant at the seedling stage only, and accessions that were susceptible at both stages. All four groups were found in each district. However, considering localities, reactions of accessions were highly variable. For example, accessions originating from Menzel Hbib were genetically variable and were represented in each of the four groups, whereas accessions from Sidi El Hani were all resistant at both stages. Further work is needed to study the genetic variability within and between localities and to better understand the resistant accessions.
Kazakh National Agrarian University
Yerlan Dutbayev, Alexei Morgounov
Kazakhstan is among the ten largest grain exporters in the world. Winter wheat in Kazakhstan is mainly cultivated in the southern and south-eastern regions on an area of 1.5-2 million hectares, including 140-170 thousand hectares - in irrigated lands. Annual losses of wheat yield from diseases can reach up to 30-40% or more. For Kazakhstan, the most dangerous diseases of winter wheat are stripe rust and leaf rust. Work is under way in Kazakhstan to find new donors for resistance to leaf rust and stripe rust and the use of these donors in breeding. The aim of this research was to expand genetic diversity through crosses and development of lines obtained by the method of remote hybridization, as well as selection of new sources of resistance of bread wheat to leaf rust and stripe rust in southeast Kazakhstan. The subject of the research were 49 hexaploidsynthtic lines of Kyoto University (Japan) and CIMMYT and commercial varieties of winter wheat in the Almaty region. We screened synthetic hexaploid wheat for resistance to diseases. A collection of hexaploid synthetic wheat lines resistant to the diseases and adapted to various conditions of the Almaty region has been established. The character of inheritance of resistance to diseases in crosses of synthetic wheat with local cultivars based on comparison of the first generation and parents was studied. Evaluation of phenotypes inheritance of resistance in hybrids in the generation of F2, showed that 9crosses of synthetic wheat(LANGDON/IG 48042//ZHETISU, LANGDON/IG 48042//FARABI, LANGDON/KU-20-8//AJARLY, LANGDON/KU-2075//AJARLY, LANGDON/KU-2097// ZHETISU, LANGDON/KU-2075//FARABI, LANGDON/KU-2100//STEKLOV, LANGDON/KU-2144//NAZ, LANGDON/KU-2076//NAZ)possess the dominant resistance genes to leaf rust.Seven lines(LANGDON/ KU-2075/AJARLY, LANGDON/KU-2075/FARABI, LANGDON/KU-2092/FARABI, LANGDON/KU-2100/NAZ, LANGDON/KU-2097/STEKLOVINDAYA, LANGDON/KU-2097/ZHETISU, LANGDON/KU-2097/ AJARLY) possess from one to several dominant resistance genes to stripe rust.
Ayele Badebo, Abebe Atilaw, Habtemariam Zegeye, Zerihun Tadesse, Wasihun Legesse, Terefe Fitta, Dawit Asnake
In Ethiopia, quality seed of improved varieties is the least expensive and most critical input for the sustainable production of wheat, a strategic food security crop grown by some 4.7 million households on 1.7 million hectares. Because wheat is self-pollinated, farmers can save and replant seed from their harvests for several years, without the variety losing its genetic identity. At the same time, recommended seed rates for wheat (150 to 200 kilograms per hectare) are significantly higher than those for tef (15 kg/ha) or maize (25 kg/ha), so some 255,000 tons of seed is required to sow Ethiopia's entire wheat area each year. Most of this still comes from informal seed systems; only four seed enterprises (ESE, ASE, OSE and SNNPSE) currently produce certified seed of various crops and they lack the capacity to supply enough high quality seed for the nation's approximately 20 million households. In collaboration with the Ethiopian Institute of Agricultural Research (EIAR) and through the USAID-funded project "Seed multiplication and delivery of high-yielding rust resistant bread and durum wheat varieties to Ethiopian farmers," the International Maize and Wheat Improvement Center (CIMMYT) is working to increase wheat farmers access to affordable, certified seed of improved varieties that are high-yielding and also feature durable resistance to the rust diseases. Approaches pursued include the fast-track evaluation and release of improved varieties, the pre-release or accelerated seed multiplication of released wheat varieties through formal and informal seed systems, and demonstrations and scaling up of improved wheat varieties. This paper describes best practices to address seed shortages faced by wheat farmers in 53 woredas.
The University of Agriculture, Peshawar, Pakistan
Muhammad Khan, Muhammad Imtiaz, Zahoor Swati, Annemarie Justesen, Sajid Ali
Yellow rust caused by Puccinia striiformis is an important disease in Pakistan. The population structure of P. striiformis in the North Eastern Himalayan region of Pakistan have been shown to be genotypically diverse with potential role of sexual recombination (Ali et al., 2014b), while lesser diversity in the Southern districts of Khyber Pakhtunkhwa (KP)(Khan et al., 2015). This study was designed for the first time to assess disease status and analyze population structure of P. striiformis across three distant parts of Northwestern Pakistan i.e., Bajaur in North Western Agency and Swat and Buner in Malakand Agency, and was compared with other Pakistani populations. Depending on the intensity of infections caused by the pathogen in the tested varieties and breeding lines, the severity of the disease ranged from 5% to 100% during 2015. Yellow rust severity was the maximum on Morocco (100%), Gomal (100%) and KPWYT-18 (80%) and moderate on Ghanimat-e-IBGE (10%) and PS-2008, PS-2013, Tatara and Millat with 20% severity. A total of 81 single lesion samples collected on infected varieties were genotyped with 18 microsatellite markers. From these, 63 distinct multilocus genotypes (MLGs) were detected; 15 single lesion samples collected from Buner produced 15 distinct MLGs signifying very high diversity. A high genotypic diversity with clear signature of recombination was detected across all the three locations. Buner (100%) had the maximum diversity followed by Swat (97%) and Bajaur (91%). The observed diversity was almost equal to other Northeastern Himalayan populations of Pakistan, while it was high when compared to some southern populations of KP (genotypic diversity of 0.895) and other worldwide clonal populations (Ali et al., 2014a). The high diversity and recombinant population structure suggested potential role of sexual reproduction in these areas, which needs to be further explored to establish the origin of diverse virulence pattern in Pakistan.
University of Seville
Solis,Ignacio, , , , , , , , , , , , , , , , , , , , , , , , , , , ,
Leaf rust is an important worldwide disease on wheat caused by the fungus Puccinia triticina. Great infections on durum wheat occurred in Southern Spain in the 2000s but diminished in recent years due to deployment of resistant varieties and application of fungicides by farmers. A leaf rust survey was carried out from the 2009-15 period to monitor the virulence spectrum of the prevailing pathotypes. A total of 84 leaf rust isolates were collected on durum wheat fields. From those, single culture were obtained and used to inoculate a set of 27 differential isolines of the susceptible variety Thatcher. In addition 8 durum varieties with known Lr genes were also included.
The main highlight is that the resistance conferred by the popular Lr14a gene was broke up in 2013, but since then virulence to this gene is not widespread. In total, 23% of the isolates were virulent to the lines containing Lr14a. Lr1, Lr3, Lr3bg, Lr16, Lr24, Lr26, and Lr28 are very effective. Lines carrying Lr2c, Lr10, Lr14b, Lr20, Lr23, and LrB displayed susceptibility to most isolates. The durum varieties Jupare (Lr27+Lr31), Guayacan (Lr61), Storlom (Lr3+) and Camayo (LrCam) are also resistant against all isolates tested. Diversification of Lr genes is needed in the coming varieties to delay the appearance of new virulent races.
CREA-Research Centre for Cereal and Industrial Crops
Elisabetta Mazzucotelli, Oadi Matny, Antonietta Saccomanno, Raffaella Battaglia, Francesca Desiderio, Agata Gadaleta, Nicola Pecchioni, Pasquale De Vita, Giovanni Laido, Luigi Cattivelli, Brian Steffenson
The recent emergence of new widely virulent and aggressive strains of rusts (particularly stripe and stem rust) is threatening Italian durum wheat (Triticum turgidum L. var. durum) production, especially under the trend of higher temperature and humidity. A big effort has been undertaken to explore the genetic variability for resistance to these fungal pathogens and discovering novel resistance genes. In particular, a wide set of tetraploid wheat lines was genotyped with several thousands of SNP markers and used for association mapping. This large collection consisted of a group of durum wheat cultivars, produced from the beginning of the last century up to now, a collection of wild emmer wheats (T. dicoccoides), and lines belonging to other wild and domesticated tetraploid subspecies, as a large untapped source of genetic diversity. In a tight cooperation with the University of Minnesota, this collection was evaluated for reaction to several races of stem and stripe rust pathogens in both controlled greenhouse and field conditions. Among the genotypes belonging to the collection are parents of segregating populations which were used for the validation of mapping results. Novel resistance loci were identified, that can be incorporated into new durum varieties through breeding programs. The QTLs found in this study, together with those available in literature, were projected to the recently sequenced durum wheat genome in order to define more precisely the chromosome regions and candidate genes involved in resistance to rusts. Lines which were resistant to multiple races of rust pathogens were also found among both T. dicoccoides and durum wheat cultivars as a source of resistance genes, whose cloning will be undertaken based on the results here obtained.
This study was supported by the Italian Ministry of Foreign Affairs and International Cooperation, with the special grant RES-WHEAT.
Agharakar Research Institute Pune
yashavanthakumar,Kakanur, Vijendra, Baviskar, Ajit, Chavan, Vilas, Surve, Vijay, Khade, Juned, Bagwan, Vitthal, Gite, Shrikant, Khairanar, Sameer, Raskar, Deepak, Bankar, Satish, Misra, , , , , , , ,
MACS 3949 is a durum wheat variety developed at Agharkar Research Institute, Pune derived through selection method from 39th IDYN (CIMMYT). The variety was identified by 55th All India Wheat and Barley workshop CCS HAU, Hissar and subsequently notified by Central Sub Committee on Crop Standards, India. On the basis of mean of three years (2013-14, 2014-15 and 2015-16) data from All India coordinated experiment, grain yield of MACS 3949 (43.98 q/ha) was higher to all the checks Viz., NIDW 295 (39.70 q/ha) and UAS 428 (41.78 q/ha). Overall, MACS 3949 showed a yield advantage of about 10.78 % over NIDW 295 and 5.24 % over UAS 428. The important morphological traits of the variety described as, semi dwarf with average plant height around 81 (78-83) cm, medium sized strong waxy semi erect green leaves, parallel dense spikes with long spreading awns. Grains were amber colored, bold lustrous, semi hard, elliptical in shape with short brush, soft threshing at maturity and1000-grain weight was about 47 (42-53) gm. The variety has shown resistance to leaf rusts, in particularly the seedling resistance to race 77-complex of leaf rust, stem rust, leaf blight, powdery mildew, flag smut and karnal bunt under both natural and artificial screening conditions. It has high protein content (12.9 %), better nutritional quality (Zinc 40.6 ppm, Iron 38.6 ppm) with good milling quality (Test weight 81.4 kg/hl) and best cooking quality for pasta product having highest overall acceptability 7.25. The newly developed durum wheat variety MACS 3949 released for cultivation at Peninsular Zone in India, which is having rich source of nutritional pasta quality with high zinc and iron content will be a promising one for future potential of export at international market.