All BGRI Abstracts

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Epidemics of yellow and stem rust in Southern Italy 2016-2017

BGRI 2018 Poster Abstract
Mehran Patpour Global Rust Reference Center (GRRC), Aarhus University, Denmark
Mogens Støvring Hovmøller, Jens Grønbech Hansen, Annemarie Fejer Justesen, Tine Thach, Julian Rodriguez-Algab, Dave Hodson, Biagio Randazzo

In 2016, severe epidemics of yellow (stripe) rust were observed on durum and bread wheat in European regions where the diseases in the past were insignificant or absent. Stem rust was also observed at epidemic levels for the first time in more than 50 years in Europe. On Sicily, both yellow and stem rust caused epidemics on cultivated durum and bread wheat and numerous breeding lines. In 2017, surveys in farmer fields and trial monitoring were carried out in Southern Italy during April-June. A total of 61 farmer fields and 9 experimental plots were inspected and rust samples collected. Despite unfavourable weather conditions for rust development, stem rust, yellow rust and leaf rust were detected on 86%, 50% and 14% of the surveyed sites, respectively. The surveys on Sicily covered approximately 70% of the durum wheat area, and data uploaded and visualised on the Wheat Rust Toolbox. On mainland Italy and Sardinia, yellow rust was observed, and sampled from nine fields in Sardinia and two in Puglia, whereas stem rust was detected and sampled in experimental plots in Sicily, Sardinia, Puglia, Lazio and Emilia Romagna. A total of 94 samples of stem rust, 30 samples of yellow rust, and 3 rust samples from Berberis aetnensis were sent to GRRC. Preliminary results of yellow rust genotyping and race phenotyping showed prevalence of race Triticale2015. Warrior(-) and a new race (Pst'New'- First detected in 2016) were also detected. For stem rust, TTTTF and TTRTF were detected in Sicily and mainland Italy and TKTTF was identified in Sardinia. Susceptibility of major commercial durum cultivars and breeding lines suggests the need for both durable resistance breeding and systematic surveys coupled to an early warning system.

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Linkage Mapping of Stem Rust Resistance Gene(s) in Spring Wheat Line CI14275

BGRI 2018 Poster Abstract
Zennah Kosgey University of Minnesota, St. Paul, MN 55108, U.S.A
Ruth Dill-Macky, Ruth Wanyera, Sridhar Bhavani, Worku Bulbula, Matthew Rouse

Stem rust caused by Puccinia graminis f.sp. tritici (Pgt) is one of the major constraints to wheat (Triticum aestivum) production worldwide. Pgt races have rapidly evolved in several geographical regions due to the deployment of single resistance genes resulting in boom and bust cycles, hence combinations of resistance genes through pyramiding ensures durability of resistance in wheat varieties. Spring wheat line CI14275 displayed high levels of field resistance to stem rust in Kenya and USA compared to the parents in its pedigree (Thatcher, Kenya Farmer & Lee). To understand the genetics of resistance in CI14275, 114 Recombinant Inbred lines (RILs) were developed from the cross CI14275/LMPG-6 and screened for seedling response to Pgt races TTTTF, TPMKC, TRTTF, TTKSK & RTQQC. Chi-square goodness of fit tests suggested one-gene, three-genes, and four-genes segregated for response to races TTTTF, TPMKC and RTQQC, respectively. The RILs were all susceptible to races TTKSK and TRTTF. CI14275 showed intermediate low infection types only against races TPMKC (23-) and TTTTF (1+3C). Field screening of the population was completed in Kenya, Ethiopia and St. Paul where CI14275 showed high levels of resistance TMR (Kenya), 5MS (Ethiopia) and 5RMR (St. Paul) against the prevalent races in the stem rust screening platforms. LMPG-6 displayed susceptible responses ranging from 70S-90S in the three locations. 90K wheat Single Nucleotide Polymorphism (SNP) marker platform will be used to genotype parents and the population.

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Sowing seeds of prosperity

BGRI 2018 Poster Abstract
Kanan Vijayaraghavan Sathguru Management Consultants
Venugopal Chintada, Vijay Paranjape, Mansi Naithani, Aishwarya Vardhan

Nepal is an important wheat producer country in the South Asian region; with wheat being the third most important crop in the country after paddy (rice) and maize. Additionally, high-quality, disease free, processed seed is vital to establishing food security in South Asia. The Agriculture and Forestry University or AFU, located in the fertile Chitwan region of Nepal, is the only agriculture university catering to the needs of the Terai region and has the capability to provide innovative wheat seed solutions for small wheat-growing farmers. In the Delivering Genetic Gain Project or DGGW, the AFU has an active involvement in seed production, processing, and distribution. These activities play a major role in human capacity building in the country involving women empowerment, whole family participation in varietal selection and entrepreneurship for sustainable livelihood and overall development. Currently, under the DGGW?s Innovative Seed System in Nepal, AFU produces and aggregates seeds from farmers in the area and process it through a new seed processing unit, which is a cost-efficient version of machines commonly seen in larger agricultural facilities. At full capacity, the unit can operate up to 18 hours a day and process one ton of seed per hour. The unit it is also capable of processing rice and maize during other cropping seasons. By March 2017, more than 200 farmers applied to be part of the inaugural cohort of farmers trained in producing disease free wheat seed. The inaugural wheat season for the Seed Systems for Nepal Initiative has concluded successfully, with a total of 14 metric tons of disease-free wheat seed processed. The DGGW Seeds Systems for Nepal Initiative envisions to increase the number of empowered farmers next season, which commences on November, 2017.

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Building upon past successes for a continued impact on production and food security through breeding high yielding climate change resilient durum wheat varieties

BGRI 2018 Poster Abstract
Mohamed Salah Gharbi National Institute of Agriculture Research, Tunisia

Meeting food security challenges is a high priority in many developing countries. North African countries are among those with the highest per capita wheat consumption in the world and chronic grain deficits. Climate change scenarios predict decrease of rainfall and increase of temperature with negative impact on crop production and hence food security. Along with adoption of modern technologies, breeding higher yielding and more climate change resilient wheat varieties is widely seen as a tool that can sustain past yield gains and food production increases. Durum wheat production in Tunisia greatly benefited from the green revolution ingredients. Continued breeding lead to replacement of the early semi dwarf varieties with higher yielding, better disease resistant and more drought tolerant ones that have positively impacted yield at farmer and national level. Monitoring gains from increased yield potential and resistance to the most damaging foliar diseases, mainly septoria leaf blotch, leaf rust and stripe rust, showed that grain yield of recently released varieties is up to four times that of the tall late maturing landraces grown before the 1970's and up to 2.5 times that of varieties of the early years of the green revolution. Chlorophyll content, green leaf duration, deeper root development from diverse donors including wild wheat relatives and grain yield are being integrated in the breeding program for the selection of more drought and heat stress tolerant durum cultivars

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Preliminary results on stem rust disease in a winter wheat landrace population from Central and Western Asia

BGRI 2018 Poster Abstract
Kadir Akan Ahi Evran University, Agriculture Faculty, Plant Protection Department K?rsehir/Turkey
Nilofer Akci, Marta da Silva Lopes

Stem rust (Puccinia graminis f. sp. tritici) is a fungal disease that can significantly reduce wheat yields and quality. The goal of this study was to screen 281 winter bread wheat landraces genotypes for their reaction to stem rust disease in seedling and adult plant stage.
For seedling stage, the experiment was carried out under greenhouse conditions in Field Crops Central Research Institute in Ankara, Turkey during 2017 growing season. The genotypes were grown at 20?4?C under greenhouse condition and inoculated (avirulent on Sr24, 26, 27, and 31 resistance genes) with urediniospores in mineral oil suspension at Zadoks growth stage 11 or 12. After inoculation, the genotypes were incubated at 20?1?C with 100% humidity during 24 hours then at 18-25?C. Scoring took place after 14 days using a 0-4 scale. Infection types on the susceptible checks (cv. Gun-91 and Thatcher) were 3+ scores. For adult plant reactions, the genotypes were screened under natural epidemic conditions for Pgt (virulent on Sr5, 6, 7b, 8a, 8b, 9b, 9g, 10, 30, Tmp and Mcn resistance genes) in Seydiler-Kastamonu, Turkey. The materials were sown in a one-meter row with three replications. Stem rust development on each entry was scored using the modified Cobb scale (Little Club had reached 80-100S) in August 2017. Coefficients of infections were calculated and values below 20 were considered to be resistant.
Two (1%) (Seedling stage) genotypes and 15 (5%) (Adult stage) genotypes were resistant to Pgt. The resistance genotypes identified in this study can be used in breeding programs. SNP markers will be identified for stem rusts resistance identified in the landrace population.

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Existence of divergent lineages, virulence phenotypes and DNA methylation in the Canadian Puccinia striiformis population

BGRI 2018 Poster Abstract
Gurcharn Singh Brar Crop Development Centre/Department of Plant Science, University of Saskatchewan, Saskatoon, Canada
Sajid Ali, Dinah Qutob, Steve Ambrose, Ron Maclachlan, Kun Lou, Curtis Pozniak, Yong-Bi Fu, Andrew Sharpe, Randy Kutcher

Puccinia striiformis f. sp. tritici (Pst), the cause of wheat stripe rust, is one of the most important pathogens of wheat. Attempts have been made in the past to characterize the worldwide genetic structure of Pst populations, excluding Canada. Characterization of 59 isolates identified 33 races with three most common races representing half of the population and subtle differences in races of eastern and western prairies. For molecular characterization, 48 isolates were sequenced to obtain SNPs and genotyped with Pst-specific SSR markers. Isolates that were suspected of recombination based on SNP data were examined for their telia production ability as a proxy for sexual recombination. The study revealed that the majority of the population was clonal, however, not exclusively clonal, with the existence of four genetic lineages. Two lineages previously reported were identified: PstS0, representing an old northwestern-European and PstS1, an invasive warmer-temperature adapted lineage. Additionally, two new lineages, PstPr and PstS1-related, were detected that have not been reported previously. The PstPr and PstS1-related lineages produced more telia than the other lineages and had double the number of unique recombination events compared to PstS0 and PstS1. PstPr was concluded to be a sexual recombinant and an exotic incursion, which was closely associated with PstS5, PstS7 (Warrior), and PstS8 (Kranich) lineages, all of which arose by sexual recombination in the center of diversity - the Himalayan region. The total phenotypic variation in the population could not be explained solely by molecular genotypes, and a hypothesis on existence of epigenetic machinery in the Pst genome was tested. Homologs of the DNMTases class (DNMT1) were identified, providing compelling evidence of a role for DNA methylation. As a first report of DNA methylation, an average of ~5%, 5-methyl cytosine (5-hmC) in the Puccinia epigenome indicated the possibility of epigenetic regulation, which merits further investigation.

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Potential of conservation agriculture for cereal-based sustainable farming systems and scaling up in eastern Indo-Gangetic plains

BGRI 2018 Poster Abstract
Resona Simkhada Nepal Agriculture Research Council, Nepal
Dipendra Pokharel, Thakur Prasad Tiwari, Mahesh Gathala, Hari Krishna Shrestha

Conservation agricultural practices have been found to be climate and labor smart, and sustainable, agricultural production technologies. The decline in productivity, increase in the cost of cultivation, labor intensive practice affected the cereal based farming system in Nepal particularly at the Indo-Gangetic plains. SRFSI has been working in response to concerns about the sustainability of the cereal based farming system at Sunsari and Dhanusha district of Nepal. This study was conducted to assess the adoption and scaling up of conservation agriculture in addition to input usage, production, net profit, B:C ratio, labour use, etc. of CA practice in Sunsari district, eastern Indo-Gangetic plains of Nepal. The study employed structured questionnaires survey and key informant survey as the main data collection tools. Project reports were taken as secondary data. The primary data related for the semi-annual report and annual report of the SRFSI project were collected jointly by the DADO, Sunsari and RARST, Tarahara. Study revealed that farmers had several tangible advantages and getting higher productivity through these practices. This study assessed the potential of CA based practices in Rice-Wheat and Rice-Maize farming system to improve the yields, net profit for sustainability of the cereal based farming system.

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Genetic variability of drought sdaptive traits in nepalese wheat (Triticum aestivum L.) germplasm

BGRI 2018 Poster Abstract
Dipendra Pokharel Department of Agriculture, Sunsari, Nepal

Wheat (Triticum aestivum L.) is one of the major cereal crops vital for global food supply. Most of the wheat crop in developing world including that of Nepal is either grown with limited irrigation or under rainfed conditions and thus face moisture stress at one or more growth stages limiting grain yield. An experiment was carried out at the Institute of Agriculture and Animal Science, Rampur to evaluate the genetic variability of selected drought adaptive traits in Nepalese wheat germplasm. The wheat genotypes evaluated comprised of Nepalese landraces and commercial cultivars, CIMMYT (International Center for Maize and Wheat Improvement) derived advanced introduction lines and three checks with differential drought adaptability. The wheat genotypes were grown in pots (single plant) arranged in a replicated split plot design in greenhouse under two contrasting moisture regimes, optimum and moisture stressed. The genotypes were evaluated for water use, water use efficiency, relative leaf water content and biomass production. The ANOVA (Analysis of Variance) revealed significant variation between environments and among the wheat genotypes for most of the traits studied. A wide range of variability was observed for water use, water use efficiency, biomass yield and relative leaf water content in moisture stressed and non-stressed environments. Nepalese cultivar Gautam showed a number of favorable drought adaptive traits, whereas, Bhrikuti was average in this respect. Based on the scores of drought adaptive traits recently released Cultivar (cv). Vijay was characterized as drought sensitive. A number of landraces and advanced breeding lines showed high level of water use efficiency and other positive traits for drought adaptation.

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Genetic variability in bread wheat (Triticum Aestivum L. ) accessions using functional and random DNA Markers

BGRI 2018 Poster Abstract
Kachalla Kyari Mala Lake Chad Research Institute, Maiduguri, Borno State-Nigeria
Dattijo Aminu, Zakari Goji Silas Turaki, Fatima Henkrar, Udupa Sripada

The research was conducted at ICARDA, Rabat. Twenty-four accessions were obtained from LCRI for marker analysis. Wizard Genomic DNA Purification Kit was used for DNA extraction. DNA was extracted by CTAB method and quantified using 1.0 % (w/v) agarose gels. Total of 12 loci, 5 functional and 7 linked random DNA markers to the traits of interest were used. PowerMarker and DARwin software were used to calculate the No. of alleles and values of genetic diversity, PIC, genetic distance, and NJ dendrogram. The total No. of detected alleles was 39; and mean No. of alleles was 3.25. No. of alleles range from 1 (Dreb-B1) to 9 (Xgwm577). Genetic diversity index ranged from 0.0000 in Dreb-B1 to 0.8471 in Xgwm577. The PIC value was also varied from 0.0000 (Dreb-B1) to 0.8296 (Xgwm577). The frequency of biotic resistance linked random DNA marker allele at Xgwm144 and Xwmc44, associated with yellow and leaf rust gene was 25% each. Marker alleles Xgwm577 and Xgwm533 linked to Stb2 and Stb8 at 150 and 120bp have frequencies of 21 and 4%. The frequency of abiotic resistance showed 50% of accessions had 1R segment (1BL.1RS translocation) and 58% of accessions showed presence of 120bp allele of Xwmc89, associated with QTL for drought tolerant. Functional marker alleles of Dreb-B1 associated with drought tolerant genes showed alleles frequency in all accessions. Linked marker allele Xgwm111 linked to heat tolerant gene showed 17% allele frequency at 220bp. Rht1 and Rht2, the allele frequencies were 92 and 4%. 92% of the cultivars had photoperiod insensitive allele at Ppd-D1 locus. VrnA1a and VrnA1c primer pair amplified at 965, 876, and 484bp, allele frequency of 13 and 87%. Cluster analysis had grouped the accessions into 5 at a genetic distance level 0.15.

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Durum wheat adaptation and yield formation as affected by Ppd-1 photoperiod sensitivity genes

BGRI 2018 Poster Abstract
Dolors Villegas Institute of Agriculture and Food Research and Technology
Karim Ammar, Susanne Dreisigacker, Josí María Arjona, Conxita Royo

Understanding the effect of genetic factors controlling flowering time is crucial to fine-tune crop adaptation to each target environment and maximize yield.
A set of spring durum wheat inbred lines carrying all but one of the possible allelic combinations at Ppd-A1 and Ppd-B1 genes was developed through a collaboration between IRTA and CIMMYT. The collection was grown during several years at four sites at latitudes ranging from 19?N to 41?N in order to assess the effect of Ppd-1 genes on development, biomass production and allocation, as well as grain yield formation.
Environmental constraints were responsible for most of the observed variation for flowering time and yield components. Latitude was a main driver of flowering time, which was later in northern sites and associated with lower minimum temperatures before flowering. Data on environmental constraints explaining a large proportion of grains m-2 and kernel weight variation will be presented. The effect on flowering time of Ppd-A1 alleles conferring photoperiod insensitivity was enhanced at sites with average daylength before flowering lower than 12h. Ppd-A1 caused a stronger effect on flowering time than Ppd-B1, which was found responsible for differences in grains m-2, associated with longer photoperiods from double-ridge to terminal spikelet stages. These differences in grains m-2, however, did not result in higher yields due to kernel weight compensation. Late flowering genotypes carrying alleles conferring photoperiod sensitivity had greater biomass at anthesis but it did not confer superior yields. Early flowering times were associated with higher yields in autumn-sowing sites due to a large contribution to yield of current photosynthesis during grain filling. Early flowering genotypes tended to yield more due to higher kernel weights, and the interaction of allele combination x environment will be discussed in the context of using allelic information as environment-specific guideline in breeding efforts.

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