All BGRI Abstracts

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GENDER DIFFENCES IN ADOPTION OF IMPROVED WHEAT VARIETY TECHNOLOGY IN KENYA.

BGRI 2018 Poster Abstract
ANNE GICHANGI KENYA AGRICULTURAL AND LIVESTOCK RESEARCH ORGANIZATION (KALRO)
GODWIN,MACHARIA, BERNICE, NGINA, , , , , , , , , , , , , , , , , , , , , , , , , ,

Studies have shown that women farmers are worse off than the male counterparts in terms of adoption of improved varietal technology and hence they experience low productivity. This technology adoption gender gap affects agricultural development considering that women in Kenya play a significant role in agriculture and food production. The link between gender and adoption is likely to vary across cultures and over time. The hypothesis of significant gender differences in access to and use of productive resources and adoption of improved wheat varieties was tested. Based on bivariate analysis, significant differences in access and use of productive resources between men and women farmers were observed. Men were more likely to access credit, extension services, own and cultivate more lands compared to women. Similarly, women in female-headed households were less likely to access the productive resources compared to women in male-headed households. The factors that affect adoption of improved wheat varieties among smallholder farmers were analysed with a specific focus on women. In contrast to the conventional model of using gender of the household head, gender and plot levels analyses were conducted. The results show that the gender of the field owner had a negative effect on adoption of improved wheat varieties. This indicates that, men were more likely to adopt improved wheat varieties, compared to women farmers. Moreover, the level of education of the household head, household size, and access to credit and extension services were observed to significantly increase the likelihood of farmers adopting improved wheat varieties. In the same framework, female farmers in male-headed households who had access to credit were more likely to adopt improved wheat varieties while there was greater probability of adoption of improved wheat varieties among female farmers in female-headed households who had access to agriculture extension and belonged to a farmer organization

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Assessment of slow rusting of landraces of bread wheat to <em>Puccinia striiformis</em> f.sp. <em>tritici</em> under artificial field inoculation

BGRI 2018 Poster Abstract
Fedaa Alo ICARDA
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Yellow rust caused by Puccinia striiformis f.sp. tritici, is the most devastating fungal disease of wheat, especially in CWANA region. Growing cultivars with durable resistance is the most economical control measure. A field study was conducted to evaluate 500 bread wheat landraces along with the susceptible control ?Morocco? using artificial inoculation under field conditions at Tel Hadia, Syria during 2010-11 and 2011-12 growing seasons. The most prevailing yellow rust virulent race 70E214 was used for artificial inoculation. The disease scoring started when the disease severity was more than 50 % on the leaves of the susceptible check ?Morocco? and continued for four scorings at the intervals of 7 days. Slow rusting resistance was assessed based on the development of disease over time using the Area under Disease Progress Curve (AUDPC), Coefficient of Infection (CI), Final rust Severity (FRS), Infection Rate ?(r)? and Relative Resistance Index (RRI). None of the landraces showed immune reaction and 10% showed lowest values for all parameters, suggesting that resistance in these landraces was controlled by major genes. Approximately 65% of landraces were marked as having different levels of slow rusting and 25% were highly susceptible. Cluster analysis based on partial resistance parameters revealed two major clusters: Susceptible and low level of slow rusting were grouped in the first cluster; Resistant, high level and moderate level of partial resistance were grouped in the second cluster.
By comparing the results obtained from RRI and others parameters, we found that landraces with very low values for all parameters exhibited high RRI value of 9, while those that showed high, moderate and low levels of slow rusting, had RRI ranges of 8-9, 7-8 and 5-7, respectively. The landraces with maximum values from each parameter showed very low RRI values of less than 5.

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Resistance to race TKTTF of Puccinia graminis f. sp. tritici with virulence to SrTmp gene in Ethiopian bread wheat lines

BGRI 2018 Poster Abstract
Worku Bulbula Ethiopian Institute of Agricultural Research
Ashenafi,Gemechu, Habtamu, Tesfaye, Zerihun, Tadesse, Habtemariam, Zegeye, Netsanet, Bacha, Ayele, Badebo, Bekele, Abeyo, Pablo, Olivera, Matthew, Rouse, , , , , , , , , , , ,

Puccinia graminis f. sp. tritici (Pgt) is the major wheat production constraint in Ethiopia causing recurrent epidemics that resulted in the withdrawal of widely grown wheat cultivars from production. Among the current Pgt races detected in Ethiopia, TKTTF is the most frequent and has caused a severe epidemic in the south wheat growing regions (Bale and Arsi) after its first detection in 2012. Therefore, to avert the current situation, identifying sources of resistance to race TKTTF in breeding germplasm is a top priority to the National Wheat Breeding Program. Hence, 82 promising bread wheat lines including five check cultivars were evaluated in Debre Zeit in a TKTTF single race nursery for three consecutive seasons, 2014-2016. Ethiopian bread wheat cultivar Digalu was used as a spreader row and was inoculated using a single isolate of race TKTTF at different growth stages. The nursery was bounded by oat to reduce interference with any other stem rust race. The 82 lines were tested in the greenhouse at Cereal Disease Laboratory and were also tested with known diagnostic molecular markers. Twenty-nine lines displayed low levels of terminal stem rust severity in the field and low coefficient of infections. Fourty-one lines were resistant to race TKTTF at the seedling stage. Bread wheat lines resistant to TKTTF are valuable sources of resistance that can be deployed in wheat growing regions of Ethiopia prone to stem rust.

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Large scale pre-breeding efforts for broadening gene pool and genetic improvement of wheat

BGRI 2018 Poster Abstract
Sukhwinder Singh CIMMYT
Prashant,Vikram, Deepmala, Sehgal, Juan, Burgueno, Carolina, Sansaloni, Cynthia, Ortiz, Ernesto, Solis, Lulu, Ledesma, Pillar, Suaste, G, Fuentes, J, Ireta, A, Sharma, P, Srivastava, Sridhar, Bhavani, Thomas, Payne, V, Govindan

Wheat breeding programs have successfully harnessed the potential of elite germplasm pool and have contributed significantly to global food security. However, to obtain additional genetic gain, useful diversity for key traits from landraces, synthetics and wild relatives should be incorporated in breeding germplasm pool. Maladaptation and linkage drags are the bottlenecks in utilizing these exotic genepools for pre-breeding. A systematic, focused, large scale effort has been pursued at CIMMYT through a three-way cross (exotic x elite1 x elite2) population development strategy. Population was advanced through selected-bulk scheme in way to select relevant genetic diversity while maintaining large population sizes. A total of 984 advanced pre-breeding lines (PBLs) were evaluated in multiple environments for grain yield related traits, micronutrient content and diseases resistance (yellow rust, stem rust, powdery mildew, and karnal bunt). Potential useful lines for these traits have been identified. High-density genomic characterization of PBLs, parental elites and exotics was conducted through a "haplotype map" based approach, which revealed 16% (58/361) exotic specific haplotype block (HB) introgression in PBLs. Out of 58 exotic specific HBs, 12 (12/361 = 3%) were found associated with traits evaluated in the study. Three HBs, H1.28 (1A), H18.1 (6D) and H5.23 (2B) were significantly important as they showed consistent effects across environments for grain yield (1A and 6D) and yellow rust (2B). This significant contribution of exotics into PBLs opens avenues to mine and utilize their useful alleles in wheat improvement. This research describes systematic large-scale pre-breeding efforts, as proof of concept of exotic germplasm deployment to the breeding pipelines simultaneously enriching genetic knowledge through high-density genomics analysis. Genetic knowledge coupled with breeding efforts should provide substantial gain required for next generation wheat varietal improvement.

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Breeding of high yielding, rusts resistance and Zn-enriched wheat varieties for different agro-ecological zones of Pakistan

BGRI 2018 Poster Abstract
Maqsood Qamar Wheat Program, National Agricultural Research Center (NARC) Islamabad
Sikander,Khan Tanveer, Muhammad, Sohail, Muhammad, Shahzad Ahmed, Sayed, H. Abbass, Sundas, Wagar, Atiq, Rattu, Muhammad, Imtiaz, , , , , , , , , , , , , , , ,

Wheat plays a vital role in multifaceted farming system of Pakistan. Like other many other countries, Pakistan's sustainable wheat production is also continuously threatened by a number of biotic and abiotic stresses. Among the biotic stresses, three rust diseases of wheat have been the most devastating. Stem rust was effectively controlled with adoption of the semi-dwarf spring wheats of the Green Revolution. However, the threat of the evolution of Ug99 race of stem rust in East Africa and its migration to Iran cannot be neglected. The Chance of of Ug99 migrating from Iran into Pakistan, coupled with the presence of dangerous new races of stripe and leaf rusts invites enormous efforts for development of rust resistant varieties for sustainable production of the wheat in the country. In this regard the Wheat Program, NARC, Pakistan initiated an intensive breeding program with financial and technical support of USDA and CIMMYT. Diverse sources of resistance to the three rusts particularly to the stem rust race Ug99 were introduced from CIMMYT. Through the rigorous selection procedure, four rusts resistant wheat varieties (NARC 2011, Pakistan 2013, Zincol 2016 and Borlaug 2016) have been released. These varieties are also resistant to Ug99. The varieties i.e. NARC 2011, Borlaug 2016 and Zincol 2016 are performing well in irrigated areas whereas Pakistan 2013 is suitable for rainfed conditions. The variety Zincol 2016 has high Zn content (35 ppm) in grain as compared to national standard check variety (25 ppm). These varieties are not only higher yielding but also possess good grain quality and other desirable traits. A considerable quantity of seed of the varieties is already present in the national seed system and will reduce the risk of Ug99 threat.

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New QTL for leaf rust and stripe rust resistance in four bread wheat and two durum wheat mapping populations

BGRI 2018 Poster Abstract
Caixia Lan CIMMYT
Ravi,Singh, Julio, Huerta-Espino, Mandeep, Randhawa, , , , , , , , , , , , , , , , , , , , , , , ,

Wheat leaf rust (LR) and stripe rust (YR), caused by the air-borne fungi Puccinia triticina (Pt) and Puccinia striiformis f. sp. tritici (Pst), respectively, are considered the primary biotic threats to bread wheat and durum wheat production globally. Growing resistant wheat varieties is a key method of minimizing the extent of yield losses caused by these diseases. Bread wheat lines Francolin #1, Kenya Kongoni, Kundan and Sujata, and CIMMYT-derived durum wheat lines Bairds and Dunkler display an adequate level of adult plant resistance (APR) to both leaf rust and stripe rust in Mexican field environments. Six recombinant inbred line (RIL) populations developed from crosses Avocet/Francolin #1, Avocet/Kenya Kongoni, Avocet/Kundan, Avocet/Sujata, Atred#1/Bairds and Atred#1/Dunkler were phenotyped for leaf rust response at Ciudad Obregon, Mexico, and the bread wheat populations for stripe rust response at Toluca for under artificial inoculations for multiple seasons. The RIL populations and their parents were genotyped with the 50 K diversity arrays technology (DArT) sequence system and simple sequence repeat (SSR) markers. Known pleotropic APR genes Lr46/Yr29 mapped in all of six populations, and explained 7.4-65.1% and 7.7-66.1% severity variations for LR and YR across different bread wheat populations and accounted for 12.4-60.8% of LR severity variations over two durum wheat populations. In addition, several new APR loci identified on chromosomes 1AS, 1DS, 2BS, 2BL, 3D and 7BL in bread wheat and QTL on chromosome 6BL in durum wheat. Among these loci, QTL on chromosomes 1AS, 3D and 7BL might be represent new co-located/pleotropic loci conferring APR to LR and YR. RILs combining these APR loci can be used as sources of complex APR in both bread wheat and durum wheat breeding. In addition, the closely linked single nucleotide polymorphism (SNP) markers have been converted into breeder-friendly kompetitive allele specific PCR (KASP) markers and their diagnostic verified.

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Speed breeding with genomic selection to accelerate genetic gain for yield in spring wheat (Triticum aestivum)

BGRI 2018 Poster Abstract
Amy Watson University of Queensland
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Genomic selection (GS) in wheat can accelerate yield gain principally through a reduction in breeding cycle duration. A method for rapid generation advance called ?speed breeding? (SB) enables up to six generations of spring wheat per year, and could be used to accelerate breeding population development and be combined with GS in various breeding schemes to enable even further gains. SB and GS could be combined through a variety of different scenarios using single seed descent and also by applying GS to segregating populations in the glasshouse. Selected lines could then go into multi-location field trials for final selections and to obtain information for updating the prediction model. The increase in speed in these scenarios compared with field-based breeding schemes could greatly improve genetic gain for valuable target traits, such as yield. To test these hypotheses, a 260 multi-parent spring wheat population, genotyped with 8,000 DArT polymorphic markers, underwent yield trials over three years. Yield prediction accuracy was accessed using five-fold cross validation and predicting across years. Using these results, the rate of genetic gain achieved through either phenotypic selection in the field or a combination of SB and GS in the glasshouse were calculated. Results indicate that incorporating GS into SB growing systems would result in a higher rate of genetic gain compared to phenotypic or more traditional GS breeding schemes, due to the greater number of generations produced per year. This approach may be able to be coupled with multi-trait GS prediction models to increase accuracy, advance genetic gain and wheat variety development.

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Progress in breeding for biofortified wheat and identification of genomic regions for enhanced grain zinc and iron in wheat

BGRI 2018 Poster Abstract
Velu Govindan CIMMYT
Leonardo,Crespo-Hererra, Julio, Huerta, Ravi, Singh, , , , , , , , , , , , , , , , , , , , , , , ,

Malnutrition affects more than 2 billion people across the globe, particularly zinc and iron deficiency causes major health problem in developing world. The biofortified staple food crops such as wheat, is an important channel to contribute to the hidden hunger problem in low income countries. Breeding for enhanced zinc concentration in wheat was initiated by crossing high zinc sources identified among synthetic wheats, T. dicoccum, T. spelta and landraces. These crosses have resulted in wheat varieties with competitive yields and enhanced grain zinc were adapted by farmers in South Asia. CIMMYT-derived early-maturity wheat cultivar 'Zinc-Shakti' with about 40% increased zinc (+14 ppm), is now grown in eastern India through public-private partners. The two CIMMYT-derived biofortified varieties: 'WB2' and 'HPBW01' released in 2016 for northwestern plains zone of India. In Pakistan, 'Zincol' was released in 2016. The first high zinc wheat variety (Bari-Gom 33) with better resistance to wheat blast have been released in Bangladesh for commercial cultivation in 2017. Targeted crosses with increased population sizes were used to obtain superior progeny lines that have high zinc levels in combination with other essential traits. This has resulted in the incorporation of several novel alleles for grain zinc and iron in elite, high-yielding germplasm. High zinc and iron are under quantitative genetic control and further progress is possible as multiple QTL are pyramided in high yielding wheats. High-throughput, non-destructive phenotyping for grain zinc and iron using the X-ray fluorescence (XRF) analysis has facilitated the selection dramatically. Gene discovery and mapping studies leading to the utilization of markers to further improve the breeding efficiency. Rapid adoption of high zinc wheat varieties in South Asia and beyond is expected with the second wave of high zinc wheat lines with superior yield, heat and drought tolerance and resistance to rusts and other foliar diseases.

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Genetics of yield components for drought tolerant wheat (Triticum aestivum L.) genotypes

BGRI 2018 Poster Abstract
Sharmin Ashraf University of Agriculture, faisalabad
ihsan,khaliq, , , , , , , , , , , , , , , , , , , , , , , , , , , ,

Drought tolerance is a polygenic trait, with a complicated phenotype, often confused by plant phenology. Breeding for water stress is more complex since there are many types of abiotic stresses, such as drought, heat and salt. High yielding wheat genotypes viz., Miraj-06, 9452, 9469, 9272, 9277, CMS-127 and three testers Chakwal-50,
Kohistan-97 and Aas-11 were crossed in line ? tester mating design. Seed obtained from crosses was evaluated in field conditions for various agronomic traits under drought conditions. Recorded data were subjected to analysis of variance to determine the genetic variability. The data were analyzed statistically and combining ability
studies were tested using line ? tester analysis to find the relationship between different traits of wheat. High significant differences were observed among the lines and testers for yield related traits under stress conditions.

The female line 9452 proved to be best line on the basis of mean performance of traits under water stress. In case of testers, the male parent variety Chakwal-50 retained its performance in maximum number of traits closely followed by Aas-11. The cross combination 9272 ? Aas-11 proved best for attaining highest mean for most of
traits. In case of GCA effects line 9277 and tester Aas-11 proved best. The cross combinations 9277 ? Chakwal-50, 9452 ? Kohistan-97 exhibited highest SCA effects. The superior genotypes and crosses can be combined to develop new promising and improved varieties under water stress conditions.

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Two phases of an adult plant resistance response in wheat to Puccinia graminis f. sp. tritici

BGRI 2018 Poster Abstract
Howard Castelyn University of the Free State, South Africa
Nelzo,Ereful, Botma, Visser, Lesley, Boyd, Zakkie, Pretorius, , , , , , , , , , , , , , , , , , , , , ,

Adult plant resistance (APR) to stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is often conferred by multiple minor genes and has the potential to be durable. A preceding project identified two Kenyan wheat lines (W1406 and W6979) from the Genome Resource Unit (Norwich, UK) that exhibit APR to Pgt. The aim of this study was to investigate the APR response to Pgt race PTKST in W1406 and W6979 compared to 37-07, a susceptible control line. Histological investigation of inoculated flag leaf sheaths indicated a significant and quantifiable decrease in Pgt colony size in the APR lines at 120 hours post inoculation (hpi). Molecular analysis supported the observed fungal biomass decrease in the APR lines at 120 hpi. RNAseq analysis identified 169 transcripts differentially expressed in W1406 and 166 transcripts in W6979 when comparing 24 and 72 hpi to 0 hpi. In W1406 transcripts encoding putative pectinesterases, lipid-transfer proteins and leucine-rich repeat-like proteins were induced at 72 hpi. In W6979 only a corresponding putative pectinesterase encoding transcript was identified. Although the induced defence response in the two APR lines exhibited some dissimilarity, it potentially involves cell wall modification in both lines. Two independent sets of peroxidases were induced at 24 and 72 hpi in both lines, suggesting independent signalling events. Expression analysis suggests the occurrence of two phases of gene expression, one at 24 hpi and another at 72 hpi; the latter seeming to correspond to the inhibition of Pgt growth, manifesting as the observed APR phenotype.

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