All BGRI Abstracts

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GENETIC MAPPING OF SEEDLING AND ADULT PLANT RESISTANCE FOR STRIPE RUST IN SPRING BREAD WHEAT (TRITICUM AESTIVUM L.)

BGRI 2018 Poster Abstract
Yewubdar Isehtu Ethiopian Institute of Agricultural Research (EIAR)

Stripe rust caused by Puccinia striiformis f.sp.tritici, is one of the major diseases of wheat in the world. Experiments were carried out at two sites in Ethiopia (Kulumsa and Meraro) during the 2015 cropping season to evaluate the response of 198 elite bread wheat genotypes and two checks to the prevailing races of stripe rust at adult plant and seedling stage. The genetic profile of these genotypes was assessed using 13006 SNP markers and an association mapping was explored to determine marker?trait association. About 72.5% and 42.5% of the lines exhibited resistance at Kulumsa and Meraro, respectively. Out of 198 genotypes tested in the greenhouse, 31% exhibited common resistance for Kubsa and mixed stripe rust isolate. Only 8966 of the SNPs were polymorphic, only these were used for association mapping analysis. These markers spanned an average density of 3.47 cM per marker, with the poorest density on the D genome. Almost half of these markers were on known chromosomes, but had no position on the consensus map of bread wheat. Analysis of population structure revealed the existence of three clusters and the estimated genomic wide Linkage Disequilibrium (LD) decay in this study ranged from 0 to 50 cM. 53 SNPs in ten genomic regions located on wheat chromosome 1AL, 2AL, 2BL, 2DL, 3BL, 4BL, 4DL, 5AS, 7AL and 7BL were identified. Thirty nine SNP markers in five genomic regions at Kulumsa and 14 SNP markers in six genomic regions at Meraro explained more than 25.5% and 35.1% of phenotypic variability respectively. For seedling stage, 21 markers in ten genomic regions located on wheat chromosomes 1B, 2A, 2B, 3A, 3B, 4B, 4D, 5A, 6B and 7B were associated with resistant. These loci may be useful for choosing parents and incorporating new resistance genes into locally adapted cultivars.

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Detection of rust resistance in selected Zimbabwean and ICARDA bread wheat (Triticum aestivum L.) germplasm using conventional and molecular techniques

BGRI 2018 Poster Abstract
Bruce Mutari Crop Breeding Institute
Sripada Udupa, Charles Mutengwa, Peter Mavindidze

Host resistance is the most effective and economical method to minimize yield losses caused by rusts. The aim of this study was to detect the presence of resistance in 75 wheat genotypes. The presence of the genes viz. Sr2, Sr24, Lr34, Lr37, Lr46 and Lr68 was investigated using simple sequence repeat and sequence tagged site markers. Quantitative aspects of resistance to leaf rust were assessed through infection response (IR), disease severity (DS), coefficient of infection (CI), disease incidence (DI), leaf tip necrosis (Ltn) and area under disease progress curve (AUDPC) under natural epidemics. Highly significant (p <0.001) differences were observed among the genotypes for CI, DI, AUDPC and relative AUDPC (rAUDPC). Twenty genotypes exhibited high levels of adult plant resistance, recording CI less than 20% and AUDPC less than 300%, with moderately susceptible to susceptible reactions. The most frequently occurring gene was Lr46 (21%), followed by Lr68 (20%), Lr34 (19%) andLr37 (11%). The stem rust resistance gene Sr24 was absent in all the genotypes. Selection for Lr34 and Lr46 based on Ltn alone can sometimes be misleading because of its variable expression in different genetic backgrounds.

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Gone with the wind: Revisiting stem rust dispersal between southern Africa and Australia

BGRI 2018 Poster Abstract
Botma Visser Department of Plant Sciences, University of the Free State, South Africa
Marcel Meyer, Robert Park, Christopher Gilligan, Laura Burgin, Matthew Hort, David Hodson, Zacharias Pretorius

Despite being 10,000 km apart, the current study emphasizes the potential vulnerability of Australia to wind-borne Puccinia graminis f. sp. tritici (Pgt) spore introductions from southern Africa. Of four Pgt introductions into Australia since 1925, at least two (races 326-1,2,3,5,6 and 194-1,2,3,5,6) are thought to have originated from southern Africa. Microsatellite analysis of 29 Australian and South African Pgt races confirmed close genetic relationships between the majority of races in these two geographically separated populations, thus supporting previously reported phenotypic similarities. Using Lagrangian Particle Dispersion Model simulations with finely-resolved global meteorological data over a 14-year period and a three-day urediniospore survival time, the study showed that long distance dispersal of Pgt from southern Africa to Australia is possible, albeit rare. Transmission events occurred most frequently from central South Africa, but were also possible from southern South Africa and Zimbabwe; while none occurred from a representative source-location in Tanzania. Direct dispersal incursions into both the western and eastern Australian wheat belts were feasible. Together, the genetic and simulation data strongly support the hypothesis that earlier introductions of Pgt into Australia occurred through long-distance wind-dispersal across the Indian Ocean. The study thus acts as a warning of possible future Pgt dispersal events to Australia which could include members of the Ug99 race group. This emphasizes the continued need for Pgt surveillance on both continents.

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Developing an enriched wheat 2A chromosome map and mapping of Adult plant stripe rust resistance gene

BGRI 2018 Poster Abstract
Suruchi Jindal Punjab Agricultural University
Parampreet,Kaur, Preeni, Bawa, Bharat, Yadav, Ajay, Mahato, Inderjit, Yadav, Priti, Sharma, OP, Gupta, Parveen, Chhuneja, NS, Bains, Jaroslav, Dolezel, Bikram Singh, Gill, J, Khurana, NK, Singh, Kuldeep, Singh, Kelly, Eversole

Diploid A genome wheat species harbor immense genetic variability which has been targeted and proven useful in wheat crop improvement. Further, the development and deployment of sequence based markers in wheat using survey sequences from next generation sequencing has opened avenues for comparative analysis, gene transfer and marker assisted selection (MAS) using high throughput cost effective genotyping techniques. Chromosome 2A of wheat is known to harbor several economically important genes. The present study aimed at in silico identification of genes corresponding to full length cDNAs and mining of SSRs and ISBPs from 2A draft sequence assembly of Chinese Spring for marker development. In totality, 1029 primer pairs (478 gene based, 501 SSRs and 50 ISBPs) were used to screen for polymorphism in diploid A genome species i.e., T. monococcum and T. boeoticum that identified 221 polymorphic loci. Out of these, 119 markers were mapped in T. monococcum X T. boeoticum RIL population. The enriched 2A genetic map constituted 161 mapped markers with final map length of 549.6 cM. Further, the utility of this enriched genetic map was demonstrated towards the fine mapping of adult plant resistance (APR) QTL, QYrtm.pau-2A against stripe rust. Using composite interval mapping, a QTL was detected between G45 and G54 markers explaining 19% of phenotypic variance. The primer sequences of the two genic markers were used to find the scaffold of 343 kb from IWGSC WGA V0.4 data. Thirty five simple sequence repeat markers were designed from the scaffold sequence which are being used for the fine mapping of QYrtm.pau-2A.

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Summary on the pathogenic variability of wheat rusts in Lebanon over the period 2009-2017

BGRI 2018 Poster Abstract
Rola El Amil Lebanese Agricultural Research Institute
Claude de Vallavieille-Pope, Marc Leconte, Mogens Hovmøller, Kumarse Nazari

Wheat rusts, caused by the fungal pathogen Puccinia sp. are serious economic diseases of wheat worldwide. Surveillance, monitoring and new virulence identification are prerequisites for future race prediction and for effective breeding programs. Therefore, we decided to compile the endeavours done for surveillance over eight cropping seasons in Lebanon. The extensive field surveys were conducted yearly in major bread and durum wheat areas over the period 2009-2017 using the Borlaug Global Rust Initiative surveillance protocols. Over eight years, 136 locations were surveyed, 56 samples were collected from mainly stripe and stem rust, and X samples were phenotyped using a robust set of standards differentials lines used worlwide at Tel Hadya - ICARDA, 6 phenotyped at INRA - Grignon, 4 phenotyped at the Global Rust Reference Center (GRRC), until the season 2015-2016 the cereal rust laboratory at LARI became autonomous in race analysis. Six samples were genotyped. The latest phenotyping showed that pathotypes had combinations of the virulence for the widely deployed genes Yr2, Yr6, Yr7, Yr8, Yr9, Yr25 and Yr27 resembling to the aggressive strain PstS2, the invasive high temperature tolerant isolate. Resistance genes Yr1, Yr3, Yr4, Yr5, Yr10, Yr15, Yr17, Yr32, and YrSP were effective against all isolates. Race typing of the stem rust sample using the North American stem rust differential sets indicated presence of TKTTF in surveyed wheat growing areas as well as at ICARDA's research station in Terbol. Identified races have been used in field artificial inoculation of ICARDA's breeding program during the last two years. In conclusion, the races PstS2 and TKTTF were the dominant prevalent races in the country for yellow and stem rust respectively. This information could be useful for the region for better integrated disease management and wider diversification of resistance genes deployment in breeding programs.

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Genotype by sequencing for the study of population genetics in Puccinia triticina

BGRI 2018 Poster Abstract
Meriem Aoun Department of Plant Pathology, North Dakota State University, Fargo, ND
James,Kolmer, Maricelis, Acevedo, , , , , , , , , , , , , , , , , , , , , , , , , ,

Leaf rust, caused by Puccinia triticina Erikss. (Pt), is the most widespread wheat rust disease. Information on the virulence and genetic diversity of Pt is important for understanding the pathogen evolution, and thus effective management of wheat leaf rust. We used 20 Thatcher wheat near isogenic lines to study virulence diversity of 102 Pt isolates collected from tetraploid wheat, common wheat, and Triticale worldwide. Seven races were found among 57 isolates collected from tetraploid wheat while 21 races were observed among 40 common wheat type isolates. Four races were identified among the five isolates collected from Triticale. A subset of 30 Pt were genotyped using the Restriction-Associated DNA (RAD)-Genotype By Sequencing (GBS) adapted for the Ion Torrent sequencing platform. Phylogenetic analysis on 30 isolates using 2,125 SNP markers showed eight clusters supported by high bootstrap values. We observed higher genotypic diversity in common wheat type isolates compared to that in tetraploid wheat type isolates. Generally, there was a correlation between virulence phenotypes and SNP genotypes. Phylogeny results suggest that RAD-GBS is promising as a new technique for the study of population genetics in P. triticina.

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Exploring genotypic variation and assessment of stress selection indices for some productive traits in bread wheat

BGRI 2018 Poster Abstract
Muhammad Ishaq Cereal Crops Research Institute (CCRI), Pirsabak Nowshera Khyber Pakhtunkhwa-Pakistan
Gulzar,Ahmad, Imtiaz, Muhammad, Khilwat, Afridi, , , , , , , , , , , , , , , , , , , , , , , ,

In the current scenario of climatic change, exploration and development of new stable genotypes performing better under stressed and non stressed environmental conditions is the priority of wheat breeders for exploiting genetic variability to improve stress tolerant cultivars. Late planting is one of the major abiotic stresses, seriously influencing wheat production. In the current study, twenty eight bread wheat genotypes were evaluated independently under normal (optimal) and late (stress) planting conditions at Cereal Crops Research Institute (CCRI), Pirsabak Nowshera Khyber Pakhtunkhwa Pakistan during 2013-14. Analysis of variance revealed highly significant (P < 0.01) differences among the genotypes, planting (sowing dates), and genotype ? sowing dates interactions effects for the studied traits. Generally, reduction in plant height (0.41 to 10.91%) and grain yield (0.36 to 53.35%) was observed among the tested genotypes under late planting as compared to normal (optimal) planting. Least % reduction in grain yield was recorded for genotypes BWL-23 (0.36%), BWL-4(0.76%), BWL-16(1.22%) and BWL-13 (1.78%) and were found tolerant to late planting stress as compared to check (Pirsabak-2008). Eight stress selection indices i.e. Mean productivity (MP), Tolerance (TOL), Geometric Mean Productivity (GMP), Harmonic mean (HM), Stress selection Indices(SSI), Stress Tolerance Index (STI), Yield Index (YI) and Yield Stability Index (YSI) were determined based on mean performance of genotypes evaluated under normal and late planting conditions. Analysis of correlation revealed that plant height and grain yield under normal and late planting conditions, had significant positive correlation with stress selection indices i.e. GM, HM, SSI and YI. These selection indices could be effective in identification of lines/ genotypes to late planting stress tolerant conditions. Based on MP, GMP, HM, STI and YI genotypes i.e. Pirsabak-2008, BWL-23 and BWL-27 were found late planting stress tolerant and could be recommended for sowing in both normal and late planting.

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Assessment of wheat varieties and Aegilops species on yellow rust resistance in Tajikistan

BGRI 2018 Poster Abstract
Firuza Nasyrova IBPPG TAS
Anvar,Jalilov, Zubaida, Kavrakova, Menu, Mamadyusufova, Botirov, Muhiddin, , , , , , , , , , , , , , , , , , , , , ,

The wild relatives of wheat, the genus Aegilops is of great interest for breeding. Many species of the genus Aegilops are distinguished by such valuable properties as resistance to rust diseases, drought resistance, and salt tolerance.
The evaluation of local wheat varieties on resistance to yellow rust showed that local varieties showed high resistance to the pathogen and were amazed from 0 to 10 %. Evaluation of Aegilops species for resistance to yellow and brown rust, showed that the species Aegilops triunciales showed high resistance to yellow and brown rust, except Ae. triunciales, collected in the Rudaki district and showed moderate resistance to brown rust - 20%. The view of the Aegilops cylindrical collected in the Rudakinsky district showed a moderate resistance to yellow rust and a high resistance to brown rust. View Aegilops tauschii, collected in the Rudakinsky district had a high resistance to yellow and brown rust. Species Aegilops crassa to yellow and brown rust showed a reaction from moderate resistance - 30% to moderate susceptibility - 40%. As a result of the studies to assess the resistance of wheat varieties in Central Tajikistan, the cultivars Jaldak, Safedaki Gorchivin and Viyod, which had high resistance to yellow and brown rust, as well as Pamir and Surkhak varieties, showed moderate resistance.
Distinct varieties of wheat with high and moderate resistance to the pathogen of yellow rust can be used as parental forms for breeding new varieties of wheat. Studies on assessing the resistance of Aegilops species have shown that the species Aegilops triunciales has a high resistance to the pathogen of yellow rust. Species of Aegilops tauschii and Aegilops crassa, collected from the Hissar salt source, had moderate resistance to the pathogen. These species of Aegilops can be used in the selection of new varieties of wheat.

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Spreading of wheat yellow rust pathogen (Puccinia striiformis West.) in the south of Russia in 2017

BGRI 2018 Poster Abstract
Galina Vladimirovna Volkova All Russian Research Institute of Biological Plant Protection
Irina Petrovna Matveeva

Yellow rust caused by Puccinia striiformis West. is a harmful and dangerous disease in the south of Russia. Yield losses under optimum conditions on highly susceptible varieties can vary from 10 to 100%. During the growing season of 2017, cool weather with constant precipitation from the third decade of April to the first decade of June contributed to the intensive development of the pathogen. Surveys of the main winter wheat production areas in five agroclimatic zones of the region revealed that yellow rust was prevalent in all areas. The maximum development of P.striiformis was observed in southern submontane and western Priazovsky agroclimatic zones. Some varieties such as Grom, Yuka, Tanya, Anka had losses to yellow rust of up to 30-40 %. In the central and northern agroclimatic zones, the losses averaged 5%, whereas in the dry eastern steppe zone losses were only up to 1%. The build up of yellow rust inoculum in the region raises concerns that in 2018, under favorable weather conditions in spring, winter wheat crops could be infected with the disease, especially in the wetter agroclimatic zones.

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Single/multi trait genome-wide association and SNP effect estimation revealed complex architecture of rust resistance in 2300 wheat accessions

BGRI 2018 Poster Abstract
Reem Joukhadar La Trobe University
Antony Gendall, Hans Daetwyler, Matthew Hayden

Wheat stem (Sr), leaf (Lr) and stripe (Yr) rust pathogens are among the most destructive fungal diseases threatening global wheat production. We utilized 2300 wheat accession including worldwide landraces, cultivars, breeding materials and 341 synthetic accessions backcrossed with three widely grown Australian cultivars (Annuello, Yitpi and Correll) to investigate rust resistance under wide environmental conditions. The germplasm was genotyped with 90K SNP chip, and was phenotyped for two seasons in three different environments against Sr and Lr and in four different environments against Yr. Different environments for each trait showed significant correlation with mean r values of 0.53, 0.23 and 0.66 for Lr, Sr and Yr; respectively. Single-trait genome wide association (GWAS) revealed several environment-specific QTL and multi-environmental QTL distributed on all chromosomes except 6D. Multi-trait GWAS confirmed a cluster of Yr QTL on chromosome 3B (within 8.3 cM) as well as a QTL for Sr and Lr on chromosome 3D. Linkage disequilibrium and comparative mapping showed that at least three Yr QTL exists within the 3B cluster including the durable rust resistance gene Sr2/Yr30. The same region was effective against Sr resistance but did not pass the stringent significant threshold in two environments. The 3D QTL was found mainly in the synthetic germplasm with Annuello background which is known to carry the Ag. elongatum 3D translocation carrying Sr24/Lr24 resistance gene. Interestingly, estimating the SNP effect using BayesR method showed that the correlation among the highest 5% QTL effects across environments were lower than that for the small effect QTL with differences in r values of 0.25 and 0.2 for Lr and Yr respectively. These results indicate the importance of small effect QTL that cannot be captured using GWAS in achieving durable rust resistance. The detected QTL in this study are useful resources for improving bread wheat resistance to rust diseases.

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