Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-University of Bonn
Mohammad Wali,Salari, Kobra, Yusefi, Mohammad, Yusefi, Gul Mohammad, Ajir, Wakil Ahmad, Sarhadi, Jens, L?on, , , , , , , , , , , , , , , , , ,
Bread wheat is a staple food in Afghanistan. Breeding for improving yield and its components in Afghan bread wheat without using new molecular methods such as marker-assisted selection (MAS) and quantitative trait loci (QTL) mapping approaches is difficult. Therefore study of genetic analysis by focus on yield and its components as first steps is necessary. Genetic analyses were performed on a winter wheat core collection of 20 accessions and commercial varieties sampled from different regions of Afghanistan and twenty agronomic traits were evaluated over three years under fully irrigated, rain-fed and drought treatments. Grain yield was the most important trait to water deficit and was highly correlated with other agronomic traits. The germplasm was structured into two sub-populations. Field plots of the genotypes were treated to one of three treatments including full irrigation, rain supplied and rain-sheltered. A randomized complete block design with three replicate was used every year of the trial. For every agronomic trait, variance components, heritability (h2) and genetic correlations was calculated. Results of the study showed that these genotypes may be good source for national breeding programs. The multiple statistical in this study showed that results of genetics correlation and regression analysis are same. Further analysis of these traits with additional experimental data to attain persuasive conclusion is suggested.
Michel E. Ghanem, Sarrah Ben M'Barek, Gustavo Azzimonti, Silvia Pereyra, Silvia Germán, Felix Marza, Amor Yahyaoui, Pawan Singh, Michael Baum, Hans-Joachim Braun
Based on a global network of wheat partners, precision field-based wheat phenotyping platforms are being developed with the support of the CGIAR Research Program on Wheat and co-investing national agricultural research institutes. This collaboration strategy aims to i) strengthen the quality of phenotypic data to fully exploit the potential of genomic data, ii) strategic prioritization of activities based on trait screening capacities and regional needs, iii) sharing knowledge and germplasm to accelerate superior germplasm development and dissemination, iv) development of capacities. Phenotyping activities are being conducted for wheat blast (Magnaporthe oryzae) in Bolivia, Septoria tritici blotch (STB) in durum wheat in Tunisia, and for multiple diseases (leaf rust, Fusarium head blight, and STB) in bread wheats in Uruguay. Subject to further funding, additional platforms will be implemented, to contribute to a faster development of broad genetic based resistant, high yielding wheat varieties, and complementing evaluations currently performed for diseases and heat, drought and yield potential (Kenya, Ethiopia, Turkey, Mexico).
Food and Agriculture Organization of the UN
Munira Otambekova, Bahromiddin Husenov, Alexei Morgounov
Wheat as a staple food crop in Tajikistan plays a crucial role for food security of population. However its production is threatened by number of limiting factors, and among them wheat rusts are most devastating disease.
Close collaboration of local scientists and breeders was established with International Agricultural Research Centers, including CIMMYT and ICARDA since early 2000. In the result, a number of high yielding and rust resistant varieties were released in Tajikistan that occupy presently about 40% of total wheat area.
Among the major breeding objectives selection of varieties with high resistance to wheat rusts, especially yellow rust considered as a priority task. The following new varieties originated from CIMMYT international nurseries were released in the country in past years, which bear high resistance to yellow rust: Sarvar (CHEN\AEGILOPS SQUARROSA (TAUS0//BCN/3/BAV92), Yusufi (SOROCA), Vahdat (VORONA SN079), Isfara (SW89.5181/KAUZ), Fayzbaksh (TAM200.KAUZ) and Shokiri (SHARK/F4105W2.1).
During the last three years eight new varieties were submitted for official testing, and two of them already are released in 2017 (Murodi and Durakhshon) and remaining ones are under official testing. The varieties and their origin are followings: Murodi (CHEN/AE.SQ//WEAVER/3/SSERI1), Durakhshon (ATTILA/3*BCN*2//BAV92), Kamol (PYN/BAU//LAGOS-19/3/ID800994.W/VEE), Zarnisor (CROC_1/AE.SQUARROSA(205)//BORL95/3/2*MILAN), Ganj (NAC/TH.AC//3*PVN/3/MIRLO/BUC/4/2*PASTOR), Mehrgon (SAAR/WAXWING), Sipar (FRET 2*2/4/SNI/TRAP #1/3/KAUZ*2/TRAP//KAUZ/5) and Lochin (PJN/BOW//OPATA*2/3/CROC_1/AE.SQ.(224)).
Ayub Agricultural Research Institute, Faisalabad, Pakistan
Muhammad,Idrees, Faqir, Muhammad, Arshad, Mehmood, Majid, Nadeem, Saleem-ur, Rehman, Makhdoom, Hussain, Javed, Ahmad, , , , , , , , , , , , , , , ,
Under changing climatic conditions, the emergence of new diseases or new races of existing diseases is a serious threat to global wheat production. Particularly, the presence of wheat blast in Bangladesh and stem rust race Ug99 in Iran, created a fearsome and intractable situation for Pakistan. A study was planned for monitoring and surveillance of the wheat blast and rust pathogens in wheat growing districts of Punjab, Pakistan during the cropping season 2016-17 as vigilance program. During the survey, one hundred and seventy one wheat fields of upper and central Punjab region were monitored and two types of Rusts (Leaf Rust & Yellow Rust) were recorded in varying intensity on different varieties of wheat. Out of 171 locations 86 spots were free from both types of rusts i.e. Leaf Rust & Yellow Rust, while the remaining locations were found to be infected with both leaf and yellow rust. However, all the surveyed fields were free from the stem rust infestation. Among the infected fields, 23 were infected by Leaf Rust while 63 fields were infected by Yellow Rust.The susceptible type of rust attack was noticed on old/ banned/ unapproved wheat varieties. Moderately resistant to resistant reaction was observed on newly approved varieties. The rust infected samples having S or MS type infection were collected for race analysis. Similarly, blast suspected samples were analyzed in laboratory and none of the tested samples showed the presence of wheat blast pathogen, which indicates no need to panic but vigilant in future.
Vegetable Research Institute AARI, Faisalabad, Pakistan.
Etlas,Amin, , , , , , , , , , , , , , , , , , , , , , , , , , , ,
In the present study five bread wheat genotypes (9797, 9801, 9802, Chakwal-50 and Chakwal-86) were tested in a 5?5 full diallel analysis for the estimation of combining ability for yield and its related traits. In randomized complete block design (RCBD) twenty F1s along with their parents were planted in field with three replications in the research area of Department of Plant Breeding and Genetics, University of Agriculture, during 2014-15. Plant height, No. of grains/spike, spike length, No. of productive tillers/plant, flag leaf area, No. of spikelets/spike, 1000 grain weight and grain yield per plant were studied. Except spike length mean squares due to GCA were highly significant for all the traits. All the characters showed highly significant mean squares for SCA and RCA. SCA variance was lower than GCA variance for number of grains/spike and spike length presenting the major role of additive gene action in the inheritance of these traits. While for plant height, flag leaf area, number of spikelets/spike, number of fertile tillers/plant, 1000 grain weight and grain yield/plant the value of GCA variance was lower than the value of SCA variance exhibiting non-additive gene action. Chakwal-50 was the best general combiner for plant height, spike length, number of spikelets/spike, number of grains/spike and grain yield/plant. The best specific combination for most of the traits was 9802?Chakwal-86. In future wheat breeding research programmes, good specific and general combiners can be exploited.
University of Minnesota
Bedada,Girma, Bekele, Hundie, Endale, Hailu, Getaneh, Wonderufael, Bekele, Abeyo, Ayele, Badebo, Pablo, Olivera, Yue, Jin, Gordon, Cisar, Matthew, Rouse, , , , , , , , , ,
Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a significant disease limiting wheat yield in Ethiopia. Wheat varieties such as 'Digalu' with single major-effect stem rust resistance genes have not exhibited durable resistance in Ethiopia. Identifying wheat lines with adult plant resistance (APR) has been proposed as a strategy to select for durable resistance. Our objective was to test the hypothesis that APR to stem rust is non-race-specific. We selected 31 wheat lines (including 10 durum and 21 bread wheat lines) that were susceptible as seedlings to Pgt races TTKSK, TKTTF, and TRTTF. These 31 wheat lines and Digalu were evaluated in 2014 and 2015 at the Kulumsa Agricultural Research Center, Ethiopia. The lines were planted in 1 m rows and replicated twice in separate single-race-inoculated nurseries. The three single-race nurseries inoculated with Pgt races TTKSK, TKTTF, and TRTTF were separated by at least 100 m and included selective spreaders. Plot yield, thousand kernel weight (TKW), and visual disease responses were measured for each plot. We used a least-squared means test to detect differences in coefficient of infection and TKW of each line across paired race comparisons. Lines 'Park', 'CI11469', and 'CI12818' displayed significantly different coefficient of infections between races TTKSK and TRTTF. For CI11469 and CI12818, this difference was validated by significant differences in TKW. Significant differences in TKW were also detected between various race comparisons for 'ETHBW019', 'CI14798', 'CI15159', 'CI14618', and 'CI14094'. Our data demonstrated that APR in the selected germplasm was largely non-race-specific, but there were exceptions where race-specificity of APR was detected. These results have implications for resistance breeding and monitoring: testing of breeding material against prevalent Pgt races in target environments, not relying only on hotspot screening locations, and careful monitoring of deployed APR varieties are all warranted.
Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India
Vinod Kumar Mishra, Uttam Kumar, Ramesh Chand, Akhilesh Mishra, Arun Joshi
Ug99 is a devastating race of Puccinia graminis f.sp. tritici possessing virulence against resistant genes Sr31 and Sr24. This race is highly adoptive and has spread quite rapidly with 13 known variants covering 13 different countries. For reducing the vulnerability of wheat in south Asia to the Ug99, breeding durable resistant varieties is important. India, second largest wheat producer, falls in the predicted pathway of Ug99. Most of the Indian germplasm possesses Sr31 and Sr24 in their background. HUW468, a well adopted variety of north eastern plains zone (NEPZ) of India, carries durable resistance gene Sr2. To strengthen it, a MABB program was initiated to introgressed two major genes (Sr50 and SrWeb) using a donor line PMBWIR4 from CIMMYT. The foreground selection was performed with Xgwm47 for SrWeb and IB267 for Sr50 followed by the background selection by using 128 polymorphic SSR markers covering all chromosomes. Backcross progenies of HUW468 were screened in the field condition by using of Pgt race 21A-2 at IARI, Regional Station, Indore located in the central India. Superior selected lines from BC2F4:5 generation was planted at three locations in India namely; Varanasi, Indore and Dharwad. HUW468-09-25-47-09 and HUW468-09-25-47-56 were selected from BC2F5 generation having Sr50 and SrWeb along with Sr2 gene, superior agronomic performance and with 93.5% and 92.7% genome recovery, respectively. These two lines also possess 6-10 % yield superiority over the recipient parent HUW468. These lines have been submitted for registration in NBPGR (National Bureau of Plant Genetic Resources), India.
CSK Himachal Pradesh Agricultural University, Palampur, India
Aashima Bhateja, Ravi Sharma, Vijay Rana, Hanif Khan
Wheat crop is attacked by three rust diseases of which stripe rust, caused by Puccinia striiformis f. sp. tritici and leaf rust, caused by Puccinia triticina, are the most common causing greater yield losses. Thirty genotypes were studied for (APR) adult plant resistance and were evaluated in field conditions and controlled conditions. HPW 373, VW 20145, VL 3002, RKVY 231, VL 907, PBW 698 and HS 507 were found to be highly resistant to yellow rust at both seedling and adult plant stages. While, genotypes HS 490, HPW 314, HPW 360, RKVY 133, Raj 4362, DBW 113 and HPW 403 showing very low AUDPC values were found to be moderately resistant under field conditions. These lines are suggested for use in breeding program and some are in network trials for their direct release. Inheritance studies were carried out to decipher the genetics of seedling rust resistance in elite germplasm line HPW 373. The F2s were evaluated for seedling resistance against yellow rust (46S119, 78S84) and leaf rust (77-5-North American equivalent THTTM) races. Resistance in HPW 373 is controlled by single dominant gene against leaf rust (77-5) and stripe rust (78S84). Against stripe rust (46S119), resistance of HPW 373 is controlled by recessive gene. The findings are expected to contribute towards enriching diversity for leaf and stripe rust resistance in bread wheat improvement programmes.
Crop and Horticultural Science Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, Ag
Yellow (stripe) rust caused by Puccinia striiformis f. sp. tritici is the most devastating disease of bread wheat (Triticum aestivum) in the world. A wide range of virulent yellow rust pathotypes is evolving in different regions of the world causing the breakdown of widely utilized sources of resistance in wheat. Hence, the knowledge of virulence factors of pathogen and determining of effective resistance genes in the region will enable breeders to target those useful genes in their breeding programs. During cropping seasons of 2015-2016 and 2016-2017, virulence of the wheat yellow rust was investigated by planting differential cultivars and isogenic lines in a yellow rust trap nursery in Ardabil, northwest of Iran . Results showed stripe rust infections on some cultivars carrying Yr genes such as Yr1, Yr3, and Yrsp previously known to be resistant. The virulence spectrum of race population in Ardabil was identical to the Warrior race or its variants which is different from characterized races in Ardabil by carrying virulence combination for Yr1, Yr3, Yr17, Yr32, and YrSP and is avirulent on Yr8 and Yr27. Except for Yr8, Yr17 and Yr27, the common races in Ardabil are generally avirulent on Yr1, Yr3, and YrSP. This is the first report of race population in Ardabil (Iran) which is similar to the Warrior race or its variants.
Department of Agroecology, Aarhus University, Denmark
Chris Khadgi,S?rensen, Annemarie Fejer, Justesen, Mogens St?vring, Hovm?ller, , , , , , , , , , , , , , , , , , , , , , , ,
Wheat yellow (stripe) rust is a recurrent problem throughout the world, and resistant varieties are an efficient means of managing the disease. Therefore, characterization of diverse sources of resistance is of prime importance for wheat breeding. The objective of the study was to investigate variation in host response in incompatible interactions conferred by different R-genes. Epifluorescence and confocal microscopic methods were utilized for histopathological investigation of six yellow rust R-genes (Yr1, Yr5, Yr6, Yr15, Yr17 and Yr27) in Avocet S background, with Avocet S as the control. Fungal colony size and area of hypersensitive response (HR) were assessed for each interaction at 4, 8 and 16 days post inoculation (dpi). The pattern for Avocet Yr15 was distinct, because HR arrested the pathogen very early and rapidly restricted pathogen growth. Avocet Yr1 and Avocet Yr5 showed a less rapid HR and restriction of pathogen growth, but most colonies were completely surrounded by HR at 8 and 16 dpi. In Avocet Yr6 the size of colonies and the extent of HR were highly variable with continuous change up to 16 dpi. More extensive pathogen growth was observed in Avocet Yr17 and Avocet Yr27, where HR induction was delayed, resulting in large intermingled colonies at 16 dpi. All interactions were clearly different from the susceptible control. Thus each R-gene produced a different temporal and spatial distribution of fungal colonies and HR response. Colony size distributions and HR response patterns are potential parameters for characterization of host resistances with different modes of action in wheat against Pst. The results also expand our comprehension of host resistance in wheat against P. striiformis.