All BGRI Abstracts

Displaying 51 - 60 of 416 records | 6 of 42 pages

Genetic variation and differentiation in global populations of the wheat leaf rust fungus, Puccinia triticina.

BGRI 2018 Poster Abstract
James Kolmer USDA-ARS Cereal Disease Laboratory
Maria Ordonez, Silvia German, Kun Xiao, Amy Fox, Maricelis Acevedo

The leaf rust pathogen, Puccinia triticina is widespread across all major wheat growing regions worldwide. Collections of P. triticina were obtained from common and durum wheat in North America, South America, Europe, South Africa, the Middle East, East Africa, Russia, Central Asia, China, Pakistan and New Zealand in order to determine the genetic diversity within each region and genetic relationship between regions. A total of 831 single uredinial isolates were characterized for virulence to isogenic lines of Thatcher wheat and for molecular genotype at 23 SSR loci. The isolates in East Africa and Europe were the most diverse for the average number of effective alleles per locus, while the populations in Russia and North America were the least diverse. The isolates in Europe and South America had the highest number of multilocus genotypes of 81 and 75, respectively, and were the most diverse for Shannon's genotypic diversity. All populations had significantly higher levels of Ho compared to He at individual SSR loci, and had highly significant values of Ia and rd which indicated clonal reproduction. Europe had the highest number of distinct SSR genotype groups with eight, and Russia had only two SSR groups. The populations in North America and South America; Russia and Central Asia; the Middle East and East Africa; were closely related for SSR genotype based on Nei's genetic distance. Based on k means clustering and DAPC of SSR genotypes, isolates virulent to durum wheat were placed into a single separate group, and isolates virulent to common wheat were placed into five other groups. Twenty-seven SSR genotypes were found in different continental regions. Isolates with identical or highly related SSR genotypes also had identical or similar virulence, which indicated historical and current migration of P. triticina worldwide.


Response of durum wheat genotypes to rust in preliminary and regular yield trials

BGRI 2018 Poster Abstract
Iqra Ghafoor Wheat Research Institute, Ayub Agricultural Rsearch Institute Faisalabad
Amna Kanwal, Mehwish Makhdoom, Javed Ahmed, Makhdoom Hussain

Wheat is the most important cereal crop in Pakistan because it contributes major portions of daily calorie intake. Rust is an increasing threat to wheat production and ultimately food security in Asian countries. The purpose of the present study is to identify the suitable wheat lines that could significantly resist rust pathogen without compromising yield. 60 durum wheat lines, entered in preliminary and regular yield trials, were tested for various morphological and physiological traits along with adult plant disease reaction under natural rust infestation. Results indicated that there was higher incidence of yellow rust as compared to leaf rust as ten genotypes were susceptible to leaf rust. Whereas seven lines were moderately susceptible, 14 were moderately resistant and two were completely susceptible to yellow rust. These findings suggested that future breeding program should be directed towards the developments of resistant cultivars that could resist variable strains of rust pathogen under changing climatic conditions.


Genetics of yield components for drought tolerant wheat (Triticum aestivum L.) genotypes

BGRI 2018 Poster Abstract
Sharmin Ashraf University of Agriculture, faisalabad
ihsan khaliq

Drought tolerance is a polygenic trait, with a complicated phenotype, often confused by plant phenology. Breeding for water stress is more complex since there are many types of abiotic stresses, such as drought, heat and salt. High yielding wheat genotypes viz., Miraj-06, 9452, 9469, 9272, 9277, CMS-127 and three testers Chakwal-50,
Kohistan-97 and Aas-11 were crossed in line ? tester mating design. Seed obtained from crosses was evaluated in field conditions for various agronomic traits under drought conditions. Recorded data were subjected to analysis of variance to determine the genetic variability. The data were analyzed statistically and combining ability
studies were tested using line ? tester analysis to find the relationship between different traits of wheat. High significant differences were observed among the lines and testers for yield related traits under stress conditions.

The female line 9452 proved to be best line on the basis of mean performance of traits under water stress. In case of testers, the male parent variety Chakwal-50 retained its performance in maximum number of traits closely followed by Aas-11. The cross combination 9272 ? Aas-11 proved best for attaining highest mean for most of
traits. In case of GCA effects line 9277 and tester Aas-11 proved best. The cross combinations 9277 ? Chakwal-50, 9452 ? Kohistan-97 exhibited highest SCA effects. The superior genotypes and crosses can be combined to develop new promising and improved varieties under water stress conditions.


Global stem rust phenotyping network for wheat improvement

BGRI 2018 Poster Abstract
Sridhar Bhavani CIMMYT
Ruth Wanyera, Godwin Macharia, Ravi Singh, Ayele Babebo, Girma Bedada

An effective partnership between CIMMYT, KALRO, EIAR and Delivering Genetic Gains in Wheat (DGGW) project on global stem rust phenotyping has made a significant progress and impact on the Global wheat community in addressing the threat of Ug99 race group and other important stem rust races in the region. International stem rust phenotyping networks play a key role in evaluating global wheat germplasm from many countries and institutions: identifying new sources of resistance, pre-breeding, CIMMYT-Kenya shuttle breeding, pathogen survey and surveillance, varietal release and genomic selection. About 600,000 lines have been screened against Pgt race Ug99 and derivatives since 2005, and the screening capacity at KALRO has increased to 50,000 lines each year from over 20-25 countries and research institutions each year. The results from international nurseries show a shift to higher frequencies of lines with resistance to race Ug99 since the screening activities were initiated in 2008.
KALRO and EIAR and several national programs have a dynamic and successful breeding programs that benefit from collaboration, testing, and release of materials coming out of the CIMMYT breeding program. The release of over 15 varieties in Kenya as well as in Ethiopia and more than 90 varieties released in several countries globally over the years is a testament to the success of the program. with spillover effects of varieties released in Burundi, Rwanda, and Uganda.
CIMMYT-Kenya shuttle breeding has resulted in rapid recycling of over 2000 breeding populations each year between Mexico and Kenya to evaluate and select lines in early generations against virulent stem rust races in Kenya to ensure lines have adequate levels of resistance are advanced not only in early generations of breeding cycle but also materials in the yield trails (10,000 annually) that are later constituted as international nurseries and distributed to National programs and partners.


A new stem rust resistance locus detected in wheat variety Yalta

BGRI 2018 Poster Abstract
Davinder Singh University of Sydney
Robert,Park, , , , , , , , , , , , , , , , , , , , , , , , , , , ,

To monitor evolution and pathogenic variability of wheat stem rust pathogen (Puccina graminis f. sp. tritici) in Australia, the Australian Cereal Rust Control program regularly conducts national annual surveys. Recently, we detected a new pathotype 34-1,2,5,7 (culture # 661) virulent on stem rust resistance genes Sr5, Sr6, Sr7b, Sr9g, Sr11, Sr15 and Sr17. Although virulent on Sr11, this pathotype produced a low infection type (IT 22+C/X) on the Sr11-differential genotype Yalta, indicating that Yalta carries an uncharacterised resistance (SrY) in addition to Sr11. To characterize SrY, we screened a RIL population Yalta/W2691 (104 lines) with two pathotypes: 21-0 (avirulent on Sr11 or AA) and the newly identified 34-1,2,5,7 (virulent on Sr11 but avirulent on SrY or BB). Yalta produced low infection types, "1C" and "22+C/X" with pathotypes 21-0 and 34-1,2,5,7, respectively, whereas W2691 was susceptible to both pathotypes. The population segregated for AA/aa (35 Res: 69 Sus) and BB/bb (36 Res: 68 Sus) loci with pathotypes 21-0 and 34-1,2,5,7, respectively. The observed segregation (AA/aa and BB/bb) however failed to fit with predicted single gene 1:1 model (P<0.05) with both pathotypes. Joint segregation analysis (AA/aa vs BB/bb) also significantly deviated (P<0.01) from 1:1:1:1 (AABB:AAbb:aaBB:aabb) genetic model. It appears that population is skewed towards susceptibility in each case either by chance or differential gametic transmission as reported previously in progenies derived from crosses involving variety Yalta. The segregation pattern (AABB and aabb) with two pathotypes was, however, highly coupled apart from 13 lines, of which, 6 lines (AAbb) were susceptible with 21-0 and resistant with 34-1,2,5,7, and 7 lines (aaBB) resistant with 21-0 and susceptible with 34-1,2,5,7, showing that the two loci are linked (?2 linkage = 76.9; P<0.001) and located very close to each other. If that is the case, it may imply that SrY is common in wheats carrying Sr11. Cultivar Charter has been used in India to differentiate pathotypes virulent for Sr11, suggesting that Charter also carries a second stem rust locus (SrC) possibly corresponding with SrY. Further studies and mapping work are underway to determine the genetic relationship between SrY, SrC and Sr11.


Epidemics of yellow and stem rust in Southern Italy 2016-2017

BGRI 2018 Poster Abstract
Mehran Patpour Global Rust Reference Center (GRRC), Aarhus University, Denmark
Mogens Støvring Hovmøller, Jens Grønbech Hansen, Annemarie Fejer Justesen, Tine Thach, Julian Rodriguez-Algab, Dave Hodson, Biagio Randazzo

In 2016, severe epidemics of yellow (stripe) rust were observed on durum and bread wheat in European regions where the diseases in the past were insignificant or absent. Stem rust was also observed at epidemic levels for the first time in more than 50 years in Europe. On Sicily, both yellow and stem rust caused epidemics on cultivated durum and bread wheat and numerous breeding lines. In 2017, surveys in farmer fields and trial monitoring were carried out in Southern Italy during April-June. A total of 61 farmer fields and 9 experimental plots were inspected and rust samples collected. Despite unfavourable weather conditions for rust development, stem rust, yellow rust and leaf rust were detected on 86%, 50% and 14% of the surveyed sites, respectively. The surveys on Sicily covered approximately 70% of the durum wheat area, and data uploaded and visualised on the Wheat Rust Toolbox. On mainland Italy and Sardinia, yellow rust was observed, and sampled from nine fields in Sardinia and two in Puglia, whereas stem rust was detected and sampled in experimental plots in Sicily, Sardinia, Puglia, Lazio and Emilia Romagna. A total of 94 samples of stem rust, 30 samples of yellow rust, and 3 rust samples from Berberis aetnensis were sent to GRRC. Preliminary results of yellow rust genotyping and race phenotyping showed prevalence of race Triticale2015. Warrior(-) and a new race (Pst'New'- First detected in 2016) were also detected. For stem rust, TTTTF and TTRTF were detected in Sicily and mainland Italy and TKTTF was identified in Sardinia. Susceptibility of major commercial durum cultivars and breeding lines suggests the need for both durable resistance breeding and systematic surveys coupled to an early warning system.


Linkage Mapping of Stem Rust Resistance Gene(s) in Spring Wheat Line CI14275

BGRI 2018 Poster Abstract
Zennah Kosgey University of Minnesota, St. Paul, MN 55108, U.S.A
Ruth Dill-Macky, Ruth Wanyera, Sridhar Bhavani, Worku Bulbula, Matthew Rouse

Stem rust caused by Puccinia graminis f.sp. tritici (Pgt) is one of the major constraints to wheat (Triticum aestivum) production worldwide. Pgt races have rapidly evolved in several geographical regions due to the deployment of single resistance genes resulting in boom and bust cycles, hence combinations of resistance genes through pyramiding ensures durability of resistance in wheat varieties. Spring wheat line CI14275 displayed high levels of field resistance to stem rust in Kenya and USA compared to the parents in its pedigree (Thatcher, Kenya Farmer & Lee). To understand the genetics of resistance in CI14275, 114 Recombinant Inbred lines (RILs) were developed from the cross CI14275/LMPG-6 and screened for seedling response to Pgt races TTTTF, TPMKC, TRTTF, TTKSK & RTQQC. Chi-square goodness of fit tests suggested one-gene, three-genes, and four-genes segregated for response to races TTTTF, TPMKC and RTQQC, respectively. The RILs were all susceptible to races TTKSK and TRTTF. CI14275 showed intermediate low infection types only against races TPMKC (23-) and TTTTF (1+3C). Field screening of the population was completed in Kenya, Ethiopia and St. Paul where CI14275 showed high levels of resistance TMR (Kenya), 5MS (Ethiopia) and 5RMR (St. Paul) against the prevalent races in the stem rust screening platforms. LMPG-6 displayed susceptible responses ranging from 70S-90S in the three locations. 90K wheat Single Nucleotide Polymorphism (SNP) marker platform will be used to genotype parents and the population.


Building upon past successes for a continued impact on production and food security through breeding high yielding climate change resilient durum wheat varieties

BGRI 2018 Poster Abstract
Mohamed Salah Gharbi National Institute of Agriculture Research, Tunisia

Meeting food security challenges is a high priority in many developing countries. North African countries are among those with the highest per capita wheat consumption in the world and chronic grain deficits. Climate change scenarios predict decrease of rainfall and increase of temperature with negative impact on crop production and hence food security. Along with adoption of modern technologies, breeding higher yielding and more climate change resilient wheat varieties is widely seen as a tool that can sustain past yield gains and food production increases. Durum wheat production in Tunisia greatly benefited from the green revolution ingredients. Continued breeding lead to replacement of the early semi dwarf varieties with higher yielding, better disease resistant and more drought tolerant ones that have positively impacted yield at farmer and national level. Monitoring gains from increased yield potential and resistance to the most damaging foliar diseases, mainly septoria leaf blotch, leaf rust and stripe rust, showed that grain yield of recently released varieties is up to four times that of the tall late maturing landraces grown before the 1970's and up to 2.5 times that of varieties of the early years of the green revolution. Chlorophyll content, green leaf duration, deeper root development from diverse donors including wild wheat relatives and grain yield are being integrated in the breeding program for the selection of more drought and heat stress tolerant durum cultivars


Three years of the Precision Wheat Phenotyping Platform for diseases in Uruguay: current status and future prospects

BGRI 2018 Poster Abstract
Gustavo Azzimonti Instituto Nacional de Tecnologia Agropecuaria (INIA), Estaci?n La Estanzuela, Ruta 50, Km 11, Colonia, Uruguay
Vanesa,Domeniguini, N?stor, Gonz?lez, Richard, Garcia, Carolina, Saint-Pierre, Pawan, Singh, Mart?n, Quincke, Silvia, Pereyra, Silvia, Germ?n, , , , , , , , , , , , , ,

Since 2014 CGIAR-WHEAT Program has promoted the establishment of a network of field-based Precision Wheat Phenotyping Platforms (PWPP) to expand the existing collaborations between CIMMYT, ICARDA and National Agricultural Research System partners. The main goals are improving the quality of data collected and shared among institutions to enhance and accelerate the international wheat breeding, and promote synergism with the private sector and nongovernmental organizations. In 2015, the PWPP-Uruguay was established to test genotypes for multiple diseases: leaf rust, Fusarium head blight and Septoria tritici blotch. These diseases are phenotyped each year in separate field trials artificially inoculated with pathogen isolates identified as representatives of the pathogen regional population. Wheat material is sowed in plots with susceptible checks every 50 entries. Disease severity and other variables related to the disease development are measured using standard international scales at dates when the expression of plant resistance is optimal. In the first three years of the platform, more than 1500 genotypes were screened per year. These materials had diverse origins (more than eight institutions, public and private, from eight countries) and diverse types: from recent commercialized to ancient cultivars, advanced lines, International CIMMYT nurseries, mapping populations or panels. Highly resistant genotypes to multiple diseases could be selected. At the present time, we are developing and adopting advanced phenotyping methods, combining remote sensing and image analysis, and exploring their adaptation to breeding constraints. Also, extension activities as internships, training courses and student projects are being developed. Major future prospects are the enhancement of data and germplasm exchange between platform partners and the PWPP network and the involvement in collaborative phenotyping/genotyping breeding projects.


Existence of divergent lineages, virulence phenotypes and DNA methylation in the Canadian Puccinia striiformis population

BGRI 2018 Poster Abstract
Gurcharn Singh Brar Crop Development Centre/Department of Plant Science, University of Saskatchewan, Saskatoon, Canada
Sajid Ali, Dinah Qutob, Steve Ambrose, Ron Maclachlan, Kun Lou, Curtis Pozniak, Yong-Bi Fu, Andrew Sharpe, Randy Kutcher

Puccinia striiformis f. sp. tritici (Pst), the cause of wheat stripe rust, is one of the most important pathogens of wheat. Attempts have been made in the past to characterize the worldwide genetic structure of Pst populations, excluding Canada. Characterization of 59 isolates identified 33 races with three most common races representing half of the population and subtle differences in races of eastern and western prairies. For molecular characterization, 48 isolates were sequenced to obtain SNPs and genotyped with Pst-specific SSR markers. Isolates that were suspected of recombination based on SNP data were examined for their telia production ability as a proxy for sexual recombination. The study revealed that the majority of the population was clonal, however, not exclusively clonal, with the existence of four genetic lineages. Two lineages previously reported were identified: PstS0, representing an old northwestern-European and PstS1, an invasive warmer-temperature adapted lineage. Additionally, two new lineages, PstPr and PstS1-related, were detected that have not been reported previously. The PstPr and PstS1-related lineages produced more telia than the other lineages and had double the number of unique recombination events compared to PstS0 and PstS1. PstPr was concluded to be a sexual recombinant and an exotic incursion, which was closely associated with PstS5, PstS7 (Warrior), and PstS8 (Kranich) lineages, all of which arose by sexual recombination in the center of diversity - the Himalayan region. The total phenotypic variation in the population could not be explained solely by molecular genotypes, and a hypothesis on existence of epigenetic machinery in the Pst genome was tested. Homologs of the DNMTases class (DNMT1) were identified, providing compelling evidence of a role for DNA methylation. As a first report of DNA methylation, an average of ~5%, 5-methyl cytosine (5-hmC) in the Puccinia epigenome indicated the possibility of epigenetic regulation, which merits further investigation.