All BGRI Abstracts

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Histopathological characterization of R-gene mediated resistance to stripe rust in wheat

BGRI 2018 Poster Abstract
Kamran Saleem Department of Agroecology, Aarhus University, Denmark
Chris Khadgi,S?rensen, Annemarie Fejer, Justesen, Mogens St?vring, Hovm?ller, , , , , , , , , , , , , , , , , , , , , , , ,

Wheat yellow (stripe) rust is a recurrent problem throughout the world, and resistant varieties are an efficient means of managing the disease. Therefore, characterization of diverse sources of resistance is of prime importance for wheat breeding. The objective of the study was to investigate variation in host response in incompatible interactions conferred by different R-genes. Epifluorescence and confocal microscopic methods were utilized for histopathological investigation of six yellow rust R-genes (Yr1, Yr5, Yr6, Yr15, Yr17 and Yr27) in Avocet S background, with Avocet S as the control. Fungal colony size and area of hypersensitive response (HR) were assessed for each interaction at 4, 8 and 16 days post inoculation (dpi). The pattern for Avocet Yr15 was distinct, because HR arrested the pathogen very early and rapidly restricted pathogen growth. Avocet Yr1 and Avocet Yr5 showed a less rapid HR and restriction of pathogen growth, but most colonies were completely surrounded by HR at 8 and 16 dpi. In Avocet Yr6 the size of colonies and the extent of HR were highly variable with continuous change up to 16 dpi. More extensive pathogen growth was observed in Avocet Yr17 and Avocet Yr27, where HR induction was delayed, resulting in large intermingled colonies at 16 dpi. All interactions were clearly different from the susceptible control. Thus each R-gene produced a different temporal and spatial distribution of fungal colonies and HR response. Colony size distributions and HR response patterns are potential parameters for characterization of host resistances with different modes of action in wheat against Pst. The results also expand our comprehension of host resistance in wheat against P. striiformis.

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Puccinia striiformis population structure in Nepal and Bhutan in comparison with Himalayan population from Pakistan

BGRI 2018 Poster Abstract
Sidra Nazir The University of Agriculture, Peshawar, Pakistan
Muhammad,Khan, Sangay, Tshewang, Sarala, Lohani, David, Hodson, Muhammad, Imtiaz, Sajid, Ali, , , , , , , , , , , , , , , , , ,

The Himalayan region of Pakistan and China has been shown to be the centre of diversity of Puccinia striiformis, however, little is known about the Eastern part of the Himalayas. We studied the genetic structure of P. striiformis from Nepal and Bhutan in comparison with Pakistan through microsatellite genotyping of 66 isolates from Nepal (35 isolates) and Bhutan (31 isolates) collected during 2015 and 2016. Genetic analyses revealed a recombinant and highly diverse population structure in Bhutan and Nepal. A high level of genotypic diversity was observed for both Bhutan (0.92) and Nepal (0.67) with the detection of 53 distinct multilocus genotypes (MLGs) in the overall population; 28 for Bhutan and 27 for Nepal. Mean number of alleles per locus was higher in Bhutan (3.33) than Nepal (3.11), while the gene diversity was higher in Nepal (0.4279) than Bhutan (0.3552). A non-significant difference between the observed and the expected heterozygosity in both populations further confirmed the recombinant structure. Analyses of population subdivision revealed a low divergence between Nepal and Bhutan (FST=0.1009), along with the detection of certain common MLGs in both populations. The overall population was clearly divided into six genetic groups, with no geographical structure, confirmed by the distribution of multilocus genotypes over two countries, suggesting a potential role of migration. Comparison with the Pakistani P. striiformis population suggested a high genotypic diversity in Nepal (0.933) and Bhutan (0.959), though lower than the previously reported from Himalayan region of Pakistan (Mansehra; 0.997). The overall high diversity and recombination signature suggested the potential role of recombination in the eastern Himalayan region (Nepal and Bhutan), which needs to be considered during host resistance deployment and in the context of aerial dispersal of the pathogen.

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Evaluation of naked barley landraces from mountainous region of Nepal for yellow rust resistance.

BGRI 2018 Poster Abstract
Ajaya Karkee Nepal Agricultural Research Council, National Agriculture Genetic Resources Centre, Khumaltar, Lalitpur
Baidhya Nath,Mahto, Mina Nath, Paudel, Dhruba Bahadur, Thapa, Krishnahari, Ghimire, Bal Krishna, Joshi, Suraj, Baidya, Prem Bahadur, Magar, , , , , , , , , , , , , , , ,

Naked barley (Hordeum vulgare var. nudum L.), is an important winter crop grown in the mountain region of Nepal. Stripe rust (Puccinia striiformis f.sp. hordei), is the most destructive fungal disease of barley in the hills of Nepal with losses up to 100 %, occurring in cooler regions with higher altitude (1000-2500 m). Yield components along with final rust severity (FRS), area under disease progress curve (AUDPC) and average coefficient of infection (ACI) were evaluated for 20 indigenous barley accessions collected from mountainous region of Nepal at National Agriculture Genetic Resource Centre (Genebank), Khumaltar, Nepal during winter season of 2016-2017 with three replications. Barley cultivars displayed a range of severity from 0% to 100% with immune to susceptible reaction. AUDPC values were significantly different among the tested genotypes. Barley genotypes with accession number NGRC00837 (ACI-3), NGRC02357 (ACI-7), NGRC06026 (ACI-9) and NGRC02306 (ACI-12) were found resistant with lowest diseases progress while NGRC02350 (ACI-60), NGRC06036 (ACI-80), NGRC02312 (ACI-86), NGRC04003 (ACI-83) and NGRC02318 (ACI-93) were found as highly susceptible landraces. Correlation coefficients of agronomical parameters such as grains per spike and 1000-kernels weight with epidemiological parameters such as AUDPC and ACI were found highly significant. Resistant genotypes with low values for disease progress as well as diseases reaction were identified. The results indicate that source of resistance to yellow rust in naked barley genotypes are available in Nepal and can be used for resistant breeding in future.

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Innovative manufacturing of a cereal rust inoculation device

BGRI 2018 Poster Abstract
Zak Pretorius University of the Free State
Gerrie Booysen, Willem Boshoff, Jozua Joubert

Urediniospores of rust fungi can be applied to cereal plants in several ways. Depending on the objective and available infrastructure, plants can be inoculated with a suspension of spores in either water, light mineral oil (e.g. Soltrol 130®) or engineered fluid (e.g. Novec 7100®). Alternatively, dry spores can be allowed to settle on plant surfaces by dusting or directly applied with a spatula or small brush. Several rust laboratories employ a system where a spore-oil suspension, contained in a gelatin capsule, is sprayed onto seedling leaves by means of a dedicated atomizer connected to an air pressure source. Although this approach is easy to use and highly efficient, the devices are not commercially available in South Africa. Locally, these inoculation appliances need to be manufactured by a conventional milling process that requires a specialized workshop and skilled personnel. This subtractive process is labour intensive and greatly prohibitive in terms of costs. Using a process called Additive Manufacturing (AM), also known as "3D printing", the body of an inoculator was digitally designed and then laser sintered in nylon. Loose powder was removed from flow channels by compressed air. A copper tube fitted afterwards connected the nylon body with the spore suspension in the capsule. Replicated inoculation tests of wheat seedlings with urediniospore bulks or single pustule collections of Puccinia triticina and P. graminis f. sp. tritici resulted in consistent levels of rust severity and infection frequency. Cleaning of inoculators in acetone for 1 min followed by a 1 h heat treatment at 60°C produced no contaminant infection in follow-up tests. The design has been registered in South Africa, the USA and Europe.

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New virulence of some Puccinia triticina races to the effective wheat leaf rust resistant genes Lr 9 and Lr 19 under Egyptian field conditions

BGRI 2018 Poster Abstract
Walid El-Orabey Plant Pathology Research Institute
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Leaf rust resistance genes Lr9 and Lr19 were previously highly effective against the most predominant races of Puccinia triticina in Egypt. In 2015/2016 growing season, susceptible field reaction was recorded on these two genes where rust severity reached about 40S for Lr9 and 5S for Lr19 under Egyptian field conditions at four locations i.e. El-Behira, El-Minufiya, El-Qalubiya and El-Fayom governorates. Eight leaf rust field samples were collected from these governorates (four from each of Lr9 and Lr19). Forty single isolates were derived from the collected samples of Lr9 and Lr19 (each with 20 isolates). Eight pathotypes were identified from Lr9 and only two pathotypes were identified from Lr19. The most frequent pathotypes virulent to Lr9 were KTSPT (30%) followed by TTTMS (25%). Moreover, the other pathotypes ranged from 5 to 10%. Whereas, the most frequent pathotype virulent to Lr19 was CTTTT (85%) and the lowest PKTST was 15%. Pathotypes i.e. PRSTT, NTKTS and TTTMS identified from Lr9 were more aggressive on most of the tested leaf rust monogenic lines, as they were virulent to 36, 35 and 35 lines, respectively from a total of 39 lines. The two pathotypes; PKTST and CTTTT identified from Lr19 were virulent to 36 and 35 lines, respectively. Moreover, leaf rust pathotypes i.e. NPTNK and PRSTT from Lr9 and PKTST from Lr19 were the most aggressive on the tested wheat cultivars at seedling stage. The Lr2a was the most effective leaf rust resistance genes against the tested pathotypes at adult plant stage. Wheat cultivars Misr 1, Misr 2 and Nubariya 1 were the most resistant cultivars against the tested pathotypes at adult plant stage.

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Study of the effect of planting date on the severity of yellow rust disease on bread wheat in northeastern Syria

BGRI 2018 Poster Abstract
Omran Youssef University of Hohenheim, Germany
Afrem,Issa, Helim, Youssef, Nawzad, Suleiman, Abdul Rahman, Issa, , , , , , , , , , , , , , , , , , , , , ,

Wheat is grown in Syria during the November-December. Wheat is exposed to many strains that negatively affect its productivity especially rust diseases, which was reported on wheat in Syria for many years and the most severe in 2010, Therefore, we studied the effect of planting dates on the severity and development of yellow rust disease. Where the field trials of the 2010-2011 season were carried out at the two locations in northeastern of Syria: Al-Qamishli Research Center and Yanbouh Research Station in Al-Malekia. By cultivating the susceptible bread wheat Cham 8, where six dates were planted starting from 02.10. 2010, a difference of 15 days. The results showed there was a difference in the severity of the yellow rust disease according to the dates of cultivation and thus the stages of growth in the plant and this was evident in the Yanbouh location where the onset of the onset of injury on 08.04.2011 in the all dates and developed the infection to 40S degrees and 30%. Also, on the 24. 04. 2011, the infection was recorded at the Qamishli location only on the third and fourth dates. The disease did not develop more than 10S and 10% due to climatic conditions due to rain and high temperature during the season. The results showed a positive correlation between the evolution of the disease and vegetative growth of plants, where the growth of plants was more active at the site of Yanbouh, especially in the second, third and fourth dates in the development of infection on plants in the rest of the dates because of weak and slow growth of plants.

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Improvement of durum wheat salinity tolerance by intergeneric hybridization of Triticum durum x Hordeum marinum

BGRI 2018 Poster Abstract
Olfa Ayed-Slama National Agronomic Institute of Tunisia
FATMA,BEN JEMAA, HAJER, SLIM-AMARA, , , , , , , , , , , , , , , , , , , , , , , , , ,

Wild species with valuable genetic heritage was used long time ago in interspecific crosses to improve cultivated plants adaptation to environmental constraints. The objective of this study is to transfer the salinity tolerance of Hordeum marinum, a wild barley species, to three durum wheat varieties (Karim, Razzek and Nasr) by intergeneric crosses. In order to skip the incompatibility between these species, in vitro immature embryo rescue was performed using B5 medium (Gamborg et al., 1968). The results showed that the genotype has an important effect on the success of the crosses and the rate of regenerated plants. We have found that 34.21% of the embryos derived from hybridization Razzek x Hordeum marinum has regenerated haploid plantlets, 5.88% for Karim x Hordeum marinum cross, and 2.78% for Nasr x Hordeum marinum.
The obtained chromosomal stock of the hybrid haploid plants was doubled by colchicine treatment concentrated at 0.05%. The rate of doubled haploid plants were reduced after colchicine treatment to 26.32% for Razzek x Hordeum marinum cross, 0% for Karim crossed with Hordeum marinum and remained unchanged for Nasr x Hordeum marinum.
The doubled haploids obtained are subjected to salt stress (6-12 g/l) in order to evaluate their tolerance to salinity.

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Genomic scan in durum wheat reveals regions controlling adaptation to the heat-prone conditions of the Senegal River

BGRI 2018 Poster Abstract
Amadou Tidiane Sall ICARDA
Filippo,Bassi, Rodomiro, Ortiz, Ibrahima, Ndoye, AbdelKarim, Filali-Maltouf, Bouchra, Belkadi, Miloudi, Nachit, Michel, Baum, Hafssa, Kabbaj, Habibou, Gueye, Madiama, Cisse, , , , , , , , , ,

Wheat is a major food crop in West Africa, but its production is significantly affected by severe heat. Unfortunately, these types of high temperatures are also becoming frequent in other regions where wheat is commonly grown. In an attempt to improve durum wheat tolerance to heat, a collection of 287 elite breeding lines, including several from both ICARDA and CIMMYT, was assessed for response to heat stress in two irrigated sites along the Senegal River: Fanaye, Senegal and Kaedi, Mauritania during 2014-2015, and 2015-2016 winter seasons. The maximum recorded grain yield was 5t ha-1, which was achieved after just 90 days from sowing to harvesting. Phenological traits (heading, maturity and grain filling period) and yield components (1000-kernel weight, spike density and biomass) had also large phenotypic variation and a significant effect on grain yield performance. This panel was genotyped by 35K Axiom to generate 8,173 polymorphic SNPs. Genomic scans identified a total of 34 significant association between single nucleotide polymorphisms (SNPs) and traits across the four environments, including 15 related to phenological adaptation, 12 controlling grain yield components, and seven linked to grain yield per se. The identification of these genomic regions can now be used to design targeted crosses to pyramid heat tolerance quantitative trait loci (QTL), while the SNPs underlying these QTL can be deployed to accelerate selection process facilitated by DNA-aided breeding.

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Molecular marker assisted gene pyramiding for durable rust resistance in wheat

BGRI 2018 Poster Abstract
Shahid Nazir Agricultural Biotechnology Research Institute, Ayub Agricultural Research Institute (AARI), Faisalabad-Pakistan
Imran Habib, Sajid-ur-Rahman, Muuhammad Waqas Jamil, Muhammad Zaffar Iqbal

Rust diseases are among the most important affecting wheat because they are responsible for a significant yield reduction globally. Different types of conventional breeding approaches are currently underway to protect wheat from these diseases. The involvement of molecular genetics and biotechnology tools in conventional plant breeding sets new directions to develop crop varieties with desired traits more efficiently and accurately. An array of molecular markers linked to rust resistant genes and dense molecular genetic maps are now available for use. Marker assisted selection (MAS) is now a routine activity in various crops especially for agronomic traits that are otherwise difficult to tag like resistance to pathogens, insects, nematodes etc. Gene pyramiding involves the stacking of many genes leading to real-time expression of all genes in single variety to develop durable resistance. This method is gaining significant popularity as it would enhance the efficiency of conventional breeding methods and precise development of broad spectrum resistant capabilities. Keeping in view the significance of MAS, rust resistant wheat parental lines were selected and molecular information was tagged using gene linked markers through PCR. Conventional breeding plane was designed on the basis of molecular data and maximum crosses were made between high yielding susceptible and resistant wheat genotypes. Molecular screening and other yield parameters were keenly noted on each stage of segregating population. Three rust resistant genes i.e. Lr-34/Yr-18, Lr-46/Yr-29 and Lr-19 were successfully combined in three cross combinations. Twenty crosses were found positive for two resistant genes i.e. Lr-46/Yr-29 and Lr-19, Moreover, one cross was positive for Lr-34/Yr-18 and Lr-46/Yr-29, and one was positive for Lr-34/Yr-18 and Lr-19. Introduction of more genes is also continued to develop superior resistance against a wide range of rust pathogen in wheat.

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Screening of wheat germplasm lines for identification of sources for yellow rust resistance in Kashmir valley

BGRI 2018 Poster Abstract
Mohd Anwar Khan Sher-e-kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and kashmir, India
Reyazul Rouf Mir, Shazia Mukhtar, Rahul R., Nelwadker, M., Ashraf Bhat

In India stripe rust of wheat (Triticum aestivum L.) is important as it occurs in the severe form in North Hill Zone (NHZ) covering states of Jammu and Kashmir, Himachal Pradesh and Uttarakhand. Stripe rust thrives well under cool and moist field conditions and sometimes its epidemic is so severe that it destroys the whole crop. Although the fungicides have been applied to control this disease but their use is unfriendly to the environment and they add to the input cost of farmers. The breeding for disease resistance is an effective strategy and involves identification of stable sources of resistance and their utilization. Deployment of yellow resistance genes has helped in suppressing the intensity, effectiveness and frequency of rust epiphytotics. Many sources of yellow rust resistance exist, but these are either incompletely characterized or these have not been studied in sufficient detail needed for their designation. The present study was conducted to screen for yellow rust resistance a set of 300 wheat germplasm lines received from various national and international germplasm centers viz., CIMMYT, Mexico; CIMMYT, Ankara, Turkey; IARI sub-station, Wellington, Tamil Nadu; IIWBR, Karnal; IIWBR, Flowerdale, Shimla and SKUAST-Kashmir, Srinagar for yellow rust resistance (46S119 and 78S84 as most prevalent races) over years 2012 to 2016 under field and ployhouse conditions. The study could identify eleven wheat lines showing varying levels of resistance to yellow rust races 46S119 and 78S84 when scored at adult plant stage under both conditions. The area under disease progress curve (AUDPC) scores of the lines identified as resistant was lowest as compared to yellow rust susceptible check (Agra Local). The resistant lines identified in the study could efficiently be utilized in yellow rust breeding programmes of the country and thereby deployment of such genes over space and time for an effective and long lasting control.

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