All BGRI Abstracts

Displaying 121 - 130 of 415 records | 13 of 42 pages

Evaluation of durum wheat landraces to yellow rust (Puccinia striiformis Westendorf f. sp. tritici)

BGRI 2018 Poster Abstract
Reza Mohammadi Dryland Agricultural Research Institute, Sararood branch, AREEO, Kermanshah, Iran
Ehsan,Lorestani, Reza, Haghparast, Mohammad Reza, Jalal Kamali, Ahmed, Amri, , , , , , , , , , , , , , , , , , , , , ,

Yellow rust (Puccinia striiformis Westendorf f. sp. tritici) is an important disease on wheat worldwide and especially in the highlands of West and Central Asia. Wheat landraces are composed of complex, variable, genetically dynamic and diverse populations, in equilibrium with both biotic and abiotic stresses prevailing in their environment. A germplasm collection consisting of 380 durum wheat accessions conserved at National Plant Gene Bank (Seed and Plant Improvement Institute, Iran) with worldwide origins, along with four check varieties were screened for resistance to yellow rust, and were also evaluated for several drought adaptative traits under rainfed conditions during 2009-10 cropping season at Sararood agricultural research station, Kermanshah, Iran. The study was conducted to quantify the phenotypic diversity and exploring durum accessions for yellow rust resistance, and to characterize the agronomic profile of different subsets of accessions for reaction to local yellow rust races. High natural infection, caused by the predominant virulent races of 6E8A+ and Yr27+, was experienced as shown by the 100 S reaction of the check bread wheat ?Sardari? and several highly susceptible accessions. The tested accessions exhibited significant variation in yellow rust severity, ranging from highly resistant to highly susceptible. Approximately 12.1% of accessions were found to be resistant to yellow rust, 9.5% were moderately resistant, 10.5% were moderately susceptible and 67.9% were susceptible. The germplasm showed a relatively modest response to yellow rust as expressed by a decrease in 1000-kernel weight (TKW) and a lower yield of the susceptible vs. resistant subsets by 11.4% and 19.9%, respectively. A comparison of foreign vs. Iranian resistant accessions, revealed higher yield productivity, higher TKW, and shorter plant height for the foreign accessions. Durum germplasm may constitute valuable genetic material for breeding new durum varieties characterized by high yield productivity under rainfed conditions and with adequate resistance to yellow rust.

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Mapping resistance in wheat landraces to the Ug99 race group of the stem rust pathogen

BGRI 2018 Poster Abstract
Kerri Neugebauer USDA-ARS
Ebrahiem Babiker, Tyler Gordon, Sam Stoxen, Matthew Rouse, Yue Jin, Shiaoman Chao, John Bonman

Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a threat to wheat production worldwide. To manage this important disease, new sources of genetic resistance are needed and common wheat landraces are a potential source of such resistance. Landrace accessions from the USDA-ARS National Small Grains Collection were evaluated for seedling resistance to the Ug99 race group. To identify accessions most likely to carry novel resistance genes, a bulked segregant analysis (BSA) approach was used. Seven resistant accessions were crossed to a susceptible parent line and F3 families were tested against Pgt race TTKSK. The resistant plants were identified and grouped into two bulks per population. The bulks, along with the parents and F1 progeny, were genotyped with the 90K wheat iSelect SNP genotyping platform. Four of the populations appeared to segregate in a 1:1 phenotypic resistant/susceptible ratio, one in a 1:2 ratio, and two in 1:3 ratios. However, chi squared tests indicated the ratios were statistically the best fit for only two of the 1:1 segregating populations and one of the 1:3 segregating populations. Initial BSA results indicate the markers associated with reduced stem rust infection are located on wheat chromosomes 1DL and 2B. These mapping populations are being advanced for further evaluation to ascertain if novel resistance to the Ug99 stem rust race group is present.

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Report on rust incidence and races identified in Kenya during 2016 surveys

BGRI 2018 Poster Abstract
Ruth Wanyera Kenya Agricultural and Livestock Research Organization
Hanningtone,Wanga, Phelister, Kinyanjui, Sridhar, Bhavani, Thomas, Fetch, , , , , , , , , , , , , , , , , , , , , ,

In 2016 rust surveys were carried out in all the four key wheat growing regions: South Rift (June, July), Mount Kenya (July), North Rift (September) and Central Rift (part of August and September). A total of 304 farms were sampled. Stem rust was detected in 235 (78.3%), yellow rust in twenty-eight (9.3%) and leaf rust in fourteen (4.7%) of the farms. Stem and yellow rust were detected in all the wheat growing regions while leaf rust was detected in South, North and Central Rift. Stem rust infection ranged from TR to 90S with maximum infection in Central Rift (88.3%), Mt. Kenya region (80.3%); South Rift (76.5%) and North Rift (72.4%). Yellow rust infection ranged TR to 60S with maximum infection in Central Rift (16.7%); North Rift(13.3 %) and minimum infection in South Rift( 4.9%),) and Mt. Kenya region ( 1.7%). Leaf rust infection ranged from trace to 50S with maximum infection in North Rift (10.2%) minimum infection in Central Rift (3.3 %) and South Rift (1.2%). Fifty percent of the eight previously released wheat varieties are now susceptible to the Ug99 race. Race analysis results from AAFC Canada suggested the presence of TTKSK which was dominating in North Rift and TTKSK, TTKST and TTTTF were dominant in the screening nursery at Njoro. Yellow rust in the region has increased in the current year owing to the incursion of a probable new race AF2012 which has resulted in increased disease severity on varieties and materials tested in the International nurseries at KALRO, Njoro.

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Breeding for stripe rust resistance in spring wheat germplasm adapted to Khyber Pakhtunkhwa province of Pakistan

BGRI 2018 Poster Abstract
Fahim Ullah Khan Barani Agricultural Research Station, Kohat
Fida Mohammad, Muhammad Imtiaz

Stripe rust is one of the major limiting factors in wheat production. An objective-based breeding program was initiated at Barani Agricultural Research Station (BARS), Kohat in 2013/14 to transfer APR genes from CIMMYT and ICARDA spring wheat lines into wheat germplasm well adapted in Khyber Pakhtunkhwa (KPK). Nine high yielding but stripe rust susceptible KPK wheat varieties were crossed in various combination with 17 CIMMYT and ICARDA wheat lines carrying resistance genes. The resultant 79 F1s were backcrossed with respective susceptible parents followed by single plant selection in F2 generation. During 2015/16, 367 segregating populations/lines were screened in multi-environment stripe rust tests within Khyber Pakhtunkhwa. Sixty-nine out of 367 lines showing adequate resistance were again screened for strip rust resistance at hot spot and in yield trial at BARS, Kohat during 2016/17. Seventeen lines showed considerable resistance and were higher yielding than check cultivars. Lines exhibiting adequate resistance will be further tested in advanced yield trial at provincial and national level for possible release of new varieties in wheat.

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Global network for precision field-based wheat phenotyping

BGRI 2018 Poster Abstract
Carolina Saint Pierre CIMMYT
Michel E. Ghanem, Sarrah Ben M'Barek, Gustavo Azzimonti, Silvia Pereyra, Silvia Germán, Felix Marza, Amor Yahyaoui, Pawan Singh, Michael Baum, Hans-Joachim Braun

Based on a global network of wheat partners, precision field-based wheat phenotyping platforms are being developed with the support of the CGIAR Research Program on Wheat and co-investing national agricultural research institutes. This collaboration strategy aims to i) strengthen the quality of phenotypic data to fully exploit the potential of genomic data, ii) strategic prioritization of activities based on trait screening capacities and regional needs, iii) sharing knowledge and germplasm to accelerate superior germplasm development and dissemination, iv) development of capacities. Phenotyping activities are being conducted for wheat blast (Magnaporthe oryzae) in Bolivia, Septoria tritici blotch (STB) in durum wheat in Tunisia, and for multiple diseases (leaf rust, Fusarium head blight, and STB) in bread wheats in Uruguay. Subject to further funding, additional platforms will be implemented, to contribute to a faster development of broad genetic based resistant, high yielding wheat varieties, and complementing evaluations currently performed for diseases and heat, drought and yield potential (Kenya, Ethiopia, Turkey, Mexico).

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Sources of Resistance to Septoria Tritici Blotch Identified in Ethiopian Durum Wheat

BGRI 2018 Poster Abstract
Carlo Fadda Bioversity International
Bogale Nigir, Cherinet Alem, Yosef G. Kidane, Mario Enrico Pè, Matteo Dell'Acqua

Septoria tritici blotch (STB) is a devastating fungal disease affecting durum and bread wheat cultivation worldwide. The search for resistance sources in untapped genetic resources may speed up breeding for STB resistance. Ethiopian durum wheat landraces represent a valuable source of allelic diversity for several traits, including disease resistance. In this study, we measure STB phenotypes under natural infection on two interconnected populations: i) a diversity panel comprising 318 Ethiopian durum wheat lines, mostly farmer varieties, and ii) a nested association mapping (NAM) population developed from a subset of the diversity panel. Phenology, yield and yield component traits were concurrently measured in the populations. We evaluated the distribution of STB resistance in Ethiopian genetic materials and the relationship existing between STB resistance and agronomic traits. STB resistance sources were found in landraces as well as in NAM lines. The genetic material was genotyped with more than 13 thousand genome-wide SNP markers to describe the linkage disequilibrium and genetic structure existing within the panels. The genotyping information was combined with phenotypes to identify marker-trait associations and loci involved in STB resistance. We identified several loci, each explaining up to 10% of the phenotypic variance for disease resistance. We developed KASP markers tagging the most interesting loci to allow the uptake of our results in a breeding perspective. Our results showed that the Ethiopian untapped allelic diversity bears a great value for studying the molecular basis of STB resistance and for breeding for resistance in local and international material.

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Monitoring wheat diseases in Nepal 2014-2016

BGRI 2018 Poster Abstract
Suraj Baidya Plant Pathology Division, Nepal Agricultural Research Council
Baidya Nath Mahto, Durba Bahadur, Thapa Roshan, Basnet Nautan Raj, Gautam Sesh, Raman Upadhyaya

Disease surveillance is very important in establishing the status of disease response in crops. During the 2014 to 2016 wheat seasons, foliar blight (spot blotch caused by Bipolaris sorokiniana and tan spot caused by Pyrenophora tritici-repentis) was recorded as severe across the entire whole plains region. Foliar blight was moderate in the mid hills, especially the Kathmandu valley. Leaf rust was severe (10MS - 100S) at several places in the mid hills. This could be due either to climatic conditions or varieties susceptible to the prevailing pathotypes. Yellow rust was also recorded up to 100S in the Kathmandu valley. Newly released varieties Gaura and Dhaulagiri showed yellow rust incidence of 20MS to 40S. Stem rust was sporadic and light and was observed very late in the season (tR - 10MR) in far western districts and the Kathmandu valley. Powdery mildew was moderate and localized. Loose smut was found at low levels throughout the mid hills. In 2014, Karnal bunt (caused by Tilletia indica) was also recorded in far western regions. Five different pathotypes of P. triticina (121R63-1, 21R55, 21R63 and 0R9) and one Pst pathotype (110S119) have prevailed during the last few years. Wheat genotypes were evaluated at Khumaltar and those reputed to have Yr27, Yr27+, Yr27+Yr18, Yr31+APR, Yr9, Yr10 and Yr15 were resistant. Similarly, genotypes containing Lr34+ had lower leaf rust severities than others.

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Association of Sr2 and lesion mimic (lm) for multiple disease resistance in wheat

BGRI 2018 Poster Abstract
Sudhir Nawathe Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India
Punam Singh Yadav, Ramesh Chand, Vinod Kumar Mishra, Uttam Kumar, Arun Kumar Joshi

The Sr2 gene has been used extensively in bread wheat improvement for durable stem rust resistance. Interestingly, the resistance of Sr2, associated with the pleotrophic gene Pbc expressed as pseudo-black chaff (PBC), is tightly linked with Yr30/Lr27/Pm genes conferring multiple disease resistance. The linkage map of chromosome 3BS revealed that Sr2 is 0.43cM away from lesion mimic (lm) locus. The RIL population (Yangmai#6 ? Sonalika) of 88 lines including parents where Sonalika carries Sr2 and lm while Yangmai#6 is deficient to both was evaluated for three years (2013-2016). The objective was to determine if this fragment is inherited as one unit and provides resistance to multiple diseases. Twenty four SSR markers distributed between 0.00 to 7.09cM on 3BS covering both Sr2 (5.57cM) and lm locus (6.0cM) were studied in the RIL population. Phenotyping was done for Sr2 associated PBC and lesion mimic along with disease severity for leaf rust, and spot blotch. Positive and significant correlations were observed between leaf stem rust resistance with Sr2 carrying PBC and lm. However, lines with lm either alone or with Sr2 (showing PBC) exhibited spot blotch susceptibility. The reverse situation does not hold not true where genotypes carrying Sr2 alone showed no correlation with spot blotch resistance. This indicates that the Sr2 complex is inherited as a single unit. Use of 24 SSR also suggest that Sr2 and lm loci are tightly linked and inherited together. The co-inheritance of Sr2 and lm ensures the stability and durability of rust resistance. However, the discouraging observation of spot blotch susceptibility due to lm gene suggests a limitation in achieving multiple disease resistance in environments where spot blotch is important. We identified two transgressive segregates in the population showing least expression of lm despite the presence of Sr2 and lm together.

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SSR analysis of 2015 Puccinia graminis f. sp tritici population in Kenya

BGRI 2018 Poster Abstract
Edgar Okello University of Eldoret
Julius Ochuodho, Ruth Wanyera, Sridhar Bhavani, Les Szabo

Stem rust Ug99 and related race group are one of the major constraints of wheat production in Kenya. The challenge has been largely due to rapid evolution of races within lineage defeating resistance genes resulting in boom and burst cycles. Understanding of the pathogen population structure in major wheat growing regions in Kenya gives comprehensive information of the predominant races as well as capturing new races which may have potential of causing epidemics. Such information can have significant impact on effective gene stewardship in breeding resistant varieties. Using 11 Pgt Simple Sequence Repeats (SSR) markers we analyzed 104 single uredinial-pustule samples. Allele frequency distribution ranged from 2 to 6 per locus with an average of 3.27 per locus. Observed heterozygosity ranging from 0.297-1.000 (mean HO=?0.809) was significantly different (P< 0.001) than the expected heterozygosity (0.264 to 0.507; mean HE=?0.407) indicating that the population is asexual. Analysis of molecular variance (AMOVA) showed that the majority of the variation occurred within the samples (98%) rather than between regions (2%). Analysis of 104 samples identified 21 multiple locus genotypes (MLGs). MLG.19 was observed across the three region analyzed that is Central Rift, North Rift and Mount Kenya while MLG.18 was predominant in Mount Kenya. Based on SSR genotypes of reference isolates, Pgt clade IV (race TKTTF) was associated with MLG.16 in Central Rift Kenya while clade I (race TTKSK) had a unique MLG.10. These results indicated two main groups corresponding to Clade I (Ug99 race group) and Clade IV (race TKTTF race group). This minimum spanning network analysis pattern points to the Pgt population being asexual due to mutation. These preliminary results suggest that Pgt population in Kenya is asexual in nature. Further analysis is being conducted to ascertain geographical structure as well as compare the results with the 2011 data.

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Introgression of Sr50 and SrWeb genes in hexaploid wheat using molecular markers for enhancing stem rust resistance

BGRI 2018 Poster Abstract
Punam Singh Yadav Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India
Vinod Kumar Mishra, Uttam Kumar, Ramesh Chand, Akhilesh Mishra, Arun Joshi

Ug99 is a devastating race of Puccinia graminis f.sp. tritici possessing virulence against resistant genes Sr31 and Sr24. This race is highly adoptive and has spread quite rapidly with 13 known variants covering 13 different countries. For reducing the vulnerability of wheat in south Asia to the Ug99, breeding durable resistant varieties is important. India, second largest wheat producer, falls in the predicted pathway of Ug99. Most of the Indian germplasm possesses Sr31 and Sr24 in their background. HUW468, a well adopted variety of north eastern plains zone (NEPZ) of India, carries durable resistance gene Sr2. To strengthen it, a MABB program was initiated to introgressed two major genes (Sr50 and SrWeb) using a donor line PMBWIR4 from CIMMYT. The foreground selection was performed with Xgwm47 for SrWeb and IB267 for Sr50 followed by the background selection by using 128 polymorphic SSR markers covering all chromosomes. Backcross progenies of HUW468 were screened in the field condition by using of Pgt race 21A-2 at IARI, Regional Station, Indore located in the central India. Superior selected lines from BC2F4:5 generation was planted at three locations in India namely; Varanasi, Indore and Dharwad. HUW468-09-25-47-09 and HUW468-09-25-47-56 were selected from BC2F5 generation having Sr50 and SrWeb along with Sr2 gene, superior agronomic performance and with 93.5% and 92.7% genome recovery, respectively. These two lines also possess 6-10 % yield superiority over the recipient parent HUW468. These lines have been submitted for registration in NBPGR (National Bureau of Plant Genetic Resources), India.

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