All BGRI Abstracts

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A new warrior race of Puccinia striiformis f.sp tritici in Syria

BGRI 2018 Poster Abstract
Mohammad Kassem Aleppo University, Aleppo , Syria
Bassam,Souliman, Naem, Al-Housien, Mohammad Shafick, Hakiem, Miloudi.M, Nachit, , , , , , , , , , , , , , , , , , , , , ,

Wheat yellow rust, caused by Puccinia triticina f. sp. tritici, is the major problem in wheat production in most parts of West Asia. Monitoring of the pathogen virulence factors and their changes provides basic information for the development of an early warning system. Wheat yellow rust has become increasingly important in the Syrian central and coastal areas during the last three years, The objective of this study was to identify races of the pathogen. Yellow rust samples collected at sites in the central and the coastal plains, were analyzed on differential host genotypes with known seedling resistance genes. According to the results of race determination, races 230E150, 166E150, 230E142 and 462E128 were identified. The race 462E128 designated the Warrior race, was identified at several sites across the Syrian central plains at the end of the 2017 growing season (early and Mid-May) when yellow rust exploded suddenly on a number of varieties, despite their previous high resistance ratings. The infections rapidly reached significant levels, in spite of the high temperature (up to 33?C) and the absence of rainfall or irrigation. This new virulent race (462E128) has been able to attack wheat lines with several major resistance gene(s) including: Spaldings Prolific (SP), Yr 3+4, Triticum spelta (Yr5), which remained effective until 2016 in Syria, Virulence to lthe resistance genes Yr1, Yr2, Yr2+, Yr3V, Yr3ND, Yr4+, Yr6, Yr6+, Yr7, Yr7+, Yr9, Yr9+, Yr11, Yr12, Yr18, Yr24, Yr26 Spaldings Prolific (YrSP), Anza (YrA+) Spaldings Prolific (SP), Yr 3+4, Triticum spelta (Yr5) and Selkirk (YrSK) was also found. Virulence to Carstens V (CV), Yr 15/6* Avocet S and Yr 5/6* Avocet S; was not found. According to our findings, the Warrior race has increased in frequency within the mix of yellow rust races in these areas in Syria . It is expected that the Warrior yellow rust race will cause damage on resistant wheat cultivars in 2018.


MicroRNAs and their mega effects on gene expression in response to leaf rust in wheat (Triticum aestivum L.)

BGRI 2018 Poster Abstract
Summi Dutta Department of BioEngineering, Birla Institute of Technology, Mesra, Ranchi, India
Manish Kumar, Kunal Mukhopadhyay

Bread wheat (Triticum aestivum L.) being the world's most popular edible cereal, plays a major role in global economy. Rust in wheat leaves, caused by Puccinia triticina, affects grain quality and severely retards its production worldwide. Micro(mi)RNAs are considered major components of gene silencing and so have a great role to play during stress. The present study focuses on identification of miRNAs, produced by host to suppress pathogen as well as delivered by pathogens to encounter host defence mechanism. Therefore, these miRNAs may be called as leaf rust responsive microRNAs. Small RNA and degradome libraries were prepared from a pair of near isogenic lines of wheat (HD2329, HD2329+Lr24), one set was mock inoculated while the other set was inoculated with urediniospores of leaf rust pathogen. Using these libraries as input a vast number of miRNAs rather a population of miRNAs were identified derived from wheat that were targeting genes mostly involved in functions like defense response, signal transduction, development, metabolism, and transcriptional regulation.
When reads specifically produced under pathogen inoculation were taken as input with Puccinia triticina genome sequences as reference, only three putative miRNA precursor loci were detected and the molecules produced were called miRNA-like molecules as their precursors lacked one or two criteria essential for a true miRNA precursor. The identified miRNAs were targeting genes like F-box protein, MAP kinase, calmodulin and susceptible antioxidant protein. We further identified the presence of argonaute and dicer like domains in Puccinia proteome available at FungiEnsembl which strengthens presence of RNAi-like activities in Puccinia.
In addition, differential expression of wheat as well as Puccinia small RNAs using stem loop RT-PCR under varying time points of disease progression (0-168 hpi) revealed their direct connection with stress responses.


Virulence evolution of Puccinia striiformis f.sp. tritici on wheat in Kenya between 1970 to 1992 and 2009 to 2014

BGRI 2018 Poster Abstract
Mercy Wamalwa Egerton University Njoro, Kenya
Ruth Wanyera, James Owuoche, Julian Rodriguez, Annemarie Justesen, Lesley Lesley, Sridhar Bhavani, Cristobal Uauy, Mogens Hovmøller

Emergence of new virulent races of Puccinia striiformis f. sp. tritici (Pst) to stripe (yellow) rust resistance genes in wheat (Triticum aestivum L.) has historically resulted in severe yield losses worldwide. We conducted a study to characterize the virulence profiles of Pst races prevalent in Kenya from historic (1970-1992) and recent collections (2009-2014). Pst isolates collected during surveys in Kenya were characterized at the Global Rust Research Centre (GRRC), Denmark. Yellow rust differential sets (wheat lines with known Yr resistance genes), and strain-specific sequence-characterized-amplified-region (SCAR) markers were used to group the Pst isolates as Pst1 or Pst2. Virulence to Yr1, Yr2, Yr3,Yr6, Yr7, Yr8, Yr9, Yr17, Yr25, Yr27, and the seedling resistance in AvocetS were detected. A total of 12 virulence profiles /races were detected in isolates obtained during 1970 to 1992, while six races were detected from samples collected between 2009 to 2014. In both periods, races with virulence profiles Yr2, Yr6, Yr7, Yr8, Yr25, Yr27, Avs and Yr2, Yr6, Yr7, Yr8, Yr17, Yr25, AvS were common. The SCAR results revealed that both Pst1 and Pst2 strains were present in the Pst isolates tested, Pst1 even in isolates from the 1970s. Additional isolates were also identified with neither Pst1 nor Pst2 profiles. From our findings, race analysis is key to understand the race diversity and pre-breeding efforts for effective resistance gene deployment.


Identification and Analysis of RNA Editing Sites in the Chloroplast Transcripts of Aegilops tauschii L.

BGRI 2018 Poster Abstract
Mengxing Wang State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China
Hui,Liu, Guangwei, Xing, Xiaojun, Nie, Song, Weining, , , , , , , , , , , , , , , , , , , , , ,

RNA editing is an important way to convert cytidine (C) to uridine (U) at specific sites within RNA molecules at a post-transcriptional level in the chloroplasts of higher plants. Although it has been systematically studied in many plants, little is known about RNA editing in the wheat D genome donor Aegilops tauschii L. Here, we investigated the chloroplast RNA editing of Ae. tauschii and compared it with other wheat relatives to trace the evolution of wheat. Through bioinformatics prediction, a total of 34 C-to-U editing sites were identified, 17 of which were validated using RT-PCR product sequencing. Furthermore, 60 sites were found by the RNA-Seq read mapping approach, 24 of which agreed with the prediction and six were validated experimentally. The editing sites were biased toward tCn or nCa trinucleotides and 50-pyrimidines, which were consistent with the flanking bases of editing sites of other seed plants. Furthermore, the editing events could result in the alteration of the secondary structures and topologies of the corresponding proteins, suggesting that RNA editing might impact the function of target genes. Finally, comparative analysis found some evolutionarily conserved editing sites in wheat and two species-specific sites were also obtained. This study is the first to report on RNA editing in Aegilops tauschii L, which not only sheds light on the evolution of wheat from the point of view of RNA editing, but also lays a foundation for further studies to identify the mechanisms of C-to-U alterations.


Wheat rusts status and population structure across Pakistan during wheat growing seasons 2015-16 and 2016-17

BGRI 2018 Poster Abstract
Sajid Ali The University of Agriculture, Peshawar, Pakistan
Muhammad,Khan, Safi, Kathi, Zahoor, Swati, Manzoor, Hussain, Annemarie, Justesen, Muhamamd, Imtiaz, , , , , , , , , , , , , , , , , ,

Considering the importance of wheat rust diseases in Pakistan and the recent identification of yellow rust pathogen (Puccinia striiformis f. sp. tritici) centre of diversity in Pakistan, the present study was designed to assess the status of three wheat rusts across the country during 2015-16 and 2016-17 and analyze the population structure of P. striiformis f. sp. tritici . A total of 451 fields (from 68 districts) were surveyed during 2016 and 480 fields (from 69 districts) during 2017. A high yellow rust pressure was present during 2016 throughout Pakistan, while it was predominant only in the Northern half during 2017. Leaf rust was present in the central part of the country, while stem rust was only found in the south. In Sindh province (located in the south), yellow rust was reported unexpectedly with high severity (>60%) on varieties like Kiran and Galaxy during both the years. A set of 513 samples of P. striiformis were genotyped with microsatellite markers to assess the population diversity and spatial structure. and infer on the cause of epidemics in the Sindh province. Population genetics analyses confirmed a recombinant population structure across all locations except the Sindh province, where relatively lower diversity and lack of recombination signature was revealed. At least five genetic groups were identified in the overall population, which were found across all locations, except Sindh province where one of the genetic groups was predominant. The P. striiformis population from Sindh province with low diversity that caused unexpected epidemics in a relatively warmer region needs to be further investigated for specific adaptation traits. Our results confirmed the high diversity across Pakistan, which lies in the Himalayan centre of diversity of the pathogen. This high diversity was present in locations without the presence of alternate host (Berberis spp.) and could potentially be associated with regular migrants from the Berberis zone into the whole country.


First report of virulence to resistance genes Yrsp, Yr1 and Yr3 by wheat yellow rust pathogen (Puccinia striiformis f. sp. triti

BGRI 2018 Poster Abstract
Safarali Safavi Crop and Horticultural Science Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, Ag
Farzad Afshari

Yellow (stripe) rust caused by Puccinia striiformis f. sp. tritici is the most devastating disease of bread wheat (Triticum aestivum) in the world. A wide range of virulent yellow rust pathotypes is evolving in different regions of the world causing the breakdown of widely utilized sources of resistance in wheat. Hence, the knowledge of virulence factors of pathogen and determining of effective resistance genes in the region will enable breeders to target those useful genes in their breeding programs. During cropping seasons of 2015-2016 and 2016-2017, virulence of the wheat yellow rust was investigated by planting differential cultivars and isogenic lines in a yellow rust trap nursery in Ardabil, northwest of Iran . Results showed stripe rust infections on some cultivars carrying Yr genes such as Yr1, Yr3, and Yrsp previously known to be resistant. The virulence spectrum of race population in Ardabil was identical to the Warrior race or its variants which is different from characterized races in Ardabil by carrying virulence combination for Yr1, Yr3, Yr17, Yr32, and YrSP and is avirulent on Yr8 and Yr27. Except for Yr8, Yr17 and Yr27, the common races in Ardabil are generally avirulent on Yr1, Yr3, and YrSP. This is the first report of race population in Ardabil (Iran) which is similar to the Warrior race or its variants.


Contribution of international nurseries for selection of rust resistant wheat varieties in Tajikistan

BGRI 2018 Poster Abstract
Hafiz Muminjanov Food and Agriculture Organization of the UN
Munira Otambekova, Bahromiddin Husenov, Alexei Morgounov

Wheat as a staple food crop in Tajikistan plays a crucial role for food security of population. However its production is threatened by number of limiting factors, and among them wheat rusts are most devastating disease.
Close collaboration of local scientists and breeders was established with International Agricultural Research Centers, including CIMMYT and ICARDA since early 2000. In the result, a number of high yielding and rust resistant varieties were released in Tajikistan that occupy presently about 40% of total wheat area.
Among the major breeding objectives selection of varieties with high resistance to wheat rusts, especially yellow rust considered as a priority task. The following new varieties originated from CIMMYT international nurseries were released in the country in past years, which bear high resistance to yellow rust: Sarvar (CHEN\AEGILOPS SQUARROSA (TAUS0//BCN/3/BAV92), Yusufi (SOROCA), Vahdat (VORONA SN079), Isfara (SW89.5181/KAUZ), Fayzbaksh (TAM200.KAUZ) and Shokiri (SHARK/F4105W2.1).
During the last three years eight new varieties were submitted for official testing, and two of them already are released in 2017 (Murodi and Durakhshon) and remaining ones are under official testing. The varieties and their origin are followings: Murodi (CHEN/AE.SQ//WEAVER/3/SSERI1), Durakhshon (ATTILA/3*BCN*2//BAV92), Kamol (PYN/BAU//LAGOS-19/3/ID800994.W/VEE), Zarnisor (CROC_1/AE.SQUARROSA(205)//BORL95/3/2*MILAN), Ganj (NAC/TH.AC//3*PVN/3/MIRLO/BUC/4/2*PASTOR), Mehrgon (SAAR/WAXWING), Sipar (FRET 2*2/4/SNI/TRAP #1/3/KAUZ*2/TRAP//KAUZ/5) and Lochin (PJN/BOW//OPATA*2/3/CROC_1/AE.SQ.(224)).


Monitoring for wheat blast and rust pathogens in different agro-ecological zones of Punjab, Pakistan

BGRI 2018 Poster Abstract
Muhammad Makky Javaid Ayub Agricultural Research Institute, Faisalabad, Pakistan
Muhammad,Idrees, Faqir, Muhammad, Arshad, Mehmood, Majid, Nadeem, Saleem-ur, Rehman, Makhdoom, Hussain, Javed, Ahmad, , , , , , , , , , , , , , , ,

Under changing climatic conditions, the emergence of new diseases or new races of existing diseases is a serious threat to global wheat production. Particularly, the presence of wheat blast in Bangladesh and stem rust race Ug99 in Iran, created a fearsome and intractable situation for Pakistan. A study was planned for monitoring and surveillance of the wheat blast and rust pathogens in wheat growing districts of Punjab, Pakistan during the cropping season 2016-17 as vigilance program. During the survey, one hundred and seventy one wheat fields of upper and central Punjab region were monitored and two types of Rusts (Leaf Rust & Yellow Rust) were recorded in varying intensity on different varieties of wheat. Out of 171 locations 86 spots were free from both types of rusts i.e. Leaf Rust & Yellow Rust, while the remaining locations were found to be infected with both leaf and yellow rust. However, all the surveyed fields were free from the stem rust infestation. Among the infected fields, 23 were infected by Leaf Rust while 63 fields were infected by Yellow Rust.The susceptible type of rust attack was noticed on old/ banned/ unapproved wheat varieties. Moderately resistant to resistant reaction was observed on newly approved varieties. The rust infected samples having S or MS type infection were collected for race analysis. Similarly, blast suspected samples were analyzed in laboratory and none of the tested samples showed the presence of wheat blast pathogen, which indicates no need to panic but vigilant in future.


Mining of hulled wheat species for yield gain in bread wheat (Triticum aestivum L.)

BGRI 2018 Poster Abstract
Vikas Venu Kumaran ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, The Nilgiris, Tamilnadu, India
SIVASAMY,MURUGASAMY, JAYAPRAKASH, PARAMASIVAM, RAJESH KUMAR, MEENA, , , , , , , , , , , , , , , , , , , , , , , ,

Emmer wheat (Triticum dicoccum L.), tetraploid species (AABB) and spelt wheat (Triticum spelta L.), hexaploid species (AABBDD) are old world hulled wheat species cultivated centuries ago in different parts of the world. These species were later replaced by higher yielding bread and durum wheat in the last centuries. Grain yield is influenced by grain number per unit area and grain size which correlates positively with grain weight. Increasing the grain number was extensively and intensively explored in the past 100 years of wheat breeding which has nearly reached to saturation and leaves little room for further yield increase due to grain number?grain size trade off. Grain size/grain weight is believed to be major driving force for further improvement of wheat yield. Both the species have been characterised with larger grain size and higher grain weight; therefore an ideal source to improve the grain size/grain weight while maintaining the grain number per spike in the cultivated bread wheat. A total of 25 accessions each of emmer and spelt wheat with good grain size and weight were crossed with 5 elite bread wheat lines. In the F2 generation, recombinant lines with good grain size, higher grain weight and grains number were further backcrossed with bread wheat. Stable lines with free threshing were obtained at BC4F4 generations and were analysed for quality. Thousand grain weight (TGW) and harvest index (HI) ranged from 46-55g and 0.47-0.58 in stable lines respectively. Stable lines yielded 16-21% than the high yielding check while number of grains per spike was maintained as that of check. Stable lines involving spelt crosses have higher grain size, TGW and HI than emmer wheat crosses. Stable lines could be released directly as cultivar or else used as one of the parents in the wheat improvement programme.


GWAS of field and seedling response to individual Pgt races reveals combinations of race-specific genes in spring wheat

BGRI 2018 Poster Abstract
Erena Edae University of Minnesota
Michael Pumphrey, Matthew Rouse

Stem rust of wheat caused by the fungal pathogen Puccinia graminis f. sp. tritici historically caused major yield losses of wheat worldwide. To understand the genetic basis of stem rust resistance in contemporary North American spring wheat, genome-wide association analysis was conducted on 250 elite lines. The lines were evaluated in separate nurseries each inoculated with a different P. graminis f. sp. tritici race for three years (2013, 2015 and 2016) at Rosemount, Minnesota. The lines were also challenged with the same four races at the seedling stage in a greenhouse facility at the USDA-ARS Cereal Disease Laboratory. A total of 22,310 high-quality SNPs obtained from the Infinium 90,000 SNPs chip were used to perform association analysis. Markers strongly associated with resistance to the four races at seedling and field environments were identified. At the seedling stage, the most significant marker-trait associations were detected in the regions of known major genes (Sr6, Sr7a and Sr9b) except for race QFCSC where a strong association was detected on chromosome arm 1AL. Markers presumably linked to Sr6 and Sr7a were associated with both seedling and field resistance to specific races. A field resistance QTL on chromosome arm 2DS was detected for response to races RCRSC and TPMKC. A QTL specific to field resistance was detected for QFCSC and TPMKC on 2BL. The markers that showed strong association signals may be useful to pyramid and track race-specific stem rust resistance genes in wheat breeding programs. We postulated the presence of Sr2, Sr6, Sr7a, Sr8a, Sr9b, Sr11, Sr12, Sr24, Sr25, Sr31, and Sr57 (Lr34) in this germplasm based on phenotypic and marker data. We found that combinations of genes conferring resistance to specific P. graminis f. sp. tritici races accounts for the prevalent stem rust resistance in North American spring wheat.